Distinct Profiles of Soluble Cytokine Receptors Between B-Cell Vitreoretinal Lymphoma and Uveitis
Distinct Profiles of Soluble Cytokine Receptors Between B-Cell Vitreoretinal Lymphoma and Uveitis
复制标题
DOI：
10.1167/iovs.15-17465
复制
发表时间：
2015-11-01
影响因子：
4.4
通讯作者：
Ishibashi, Tatsuro
中科院分区：
文献类型：
--
作者：
Takeda, Atsunobu;Yoshikawa, Hiroshi;Ishibashi, Tatsuro
目的。目的：测定b细胞玻璃体视网膜淋巴瘤（VRL）和葡萄膜炎患者玻璃体中可溶性细胞因子受体和细胞因子及其主要配体的分布。玻璃体样本采集自免疫功能正常的VRL患者（n = 21）、葡萄膜炎患者（n = 20）和特发性视网膜前膜患者（n = 21）作为对照。用细胞珠法测定玻璃体中可溶性受体和细胞因子的浓度。VRL患者玻璃体中可溶性IL-2受体a （sIL-2Ra）、sIL-6R、可溶性肿瘤坏死因子受体（TNFR） 1、sTNFR2、可溶性血管内皮生长因子受体（sVEGFR） 1、sVEGFR2和IL-10水平高于葡萄膜炎患者和对照组，而葡萄膜炎患者玻璃体中sIL-1R1、sIL-1R2和sIL-4R水平高于葡萄膜炎患者和对照组。在对VRL患者的分析中，sVEGFR1和sVEGFR2水平的升高在系统性转移性视网膜淋巴瘤（SMRL）患者中比在原发性VRL/原发性中枢神经系统淋巴瘤（PVRL/PCNSL）患者中更为突出。此外，与主要为玻璃体腔混浊的VRL患者相比，发生视网膜下病变的VRL患者sIL-2Ra水平升高，与玻璃体切除细胞块中CD3（+）细胞的密度呈正相关。VRL患者的可溶性细胞因子受体和细胞因子谱与葡萄膜炎患者不同。此外，sVEGFR1和sVEGFR2水平可能是PVRL/PCNSL和SMRL之间的鉴别诊断标志物，sIL-2Ra水平可以预测VRL细胞浸润到视网膜下和/或视网膜。
PURPOSE. To determine the profiles of soluble cytokine receptors and cytokines, including mostly their ligands, in the vitreous humor of patients with B-cell vitreoretinal lymphoma (VRL) and uveitis.METHODS. Vitreous samples were collected from immunocompetent patients with VRL (n = 21), uveitis (n = 20), and idiopathic epiretinal membrane (n = 21) as controls. Cytometric beads assay were used to determine the vitreous concentrations of soluble receptors and cytokines.RESULTS. Vitreous levels of soluble IL-2 receptor a (sIL-2Ra), sIL-6R, soluble tumor necrosis factor receptor (TNFR) 1, sTNFR2, soluble vascular endothelial growth factor receptor (sVEGFR) 1, sVEGFR2, and IL-10 were higher in patients with VRL than in those with uveitis and controls, whereas those of sIL-1R1, sIL-1R2, and sIL-4R were higher in patients with uveitis than those with VRL and controls. In analyses in patients with VRL, elevation of sVEGFR1 and sVEGFR2 levels was more prominent in patients with systemic metastatic retinal lymphoma (SMRL) than in those with primary VRL/primary central nervous system lymphoma (PVRL/PCNSL). Furthermore, sIL-2Ra levels were increased in patients with VRL who developed subretinal lesions compared with in those who mainly had vitreous cavity opacity, positively correlated with the density of CD3(+) cells in the vitrectomy cell blocks.CONCLUSIONS. The profiles of soluble cytokine receptors and cytokines in patients with VRL were different from those with uveitis. In addition, sVEGFR1 and sVEGFR2 levels may be differential diagnostic markers between PVRL/PCNSL and SMRL, and sIL-2Ra levels can anticipate infiltration of VRL cells into the subretina and/or retina.