Reproductive stem cell research and its application to urology
Reproductive stem cell research and its application to urology
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DOI：
10.1111/j.1442-2042.2007.01963.x
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发表时间：
2008-02
影响因子：
2.6
通讯作者：
T. Ogawa
中科院分区：
文献类型：
--
作者：
T. Ogawa
翻译后摘要：生殖细胞的定义是他们的先天潜力，通过受精传递遗传信息给下一代。雄性长时间产生大量精子以最大限度地提高受精机会。生殖干细胞及其直接子细胞是持续产生大量精子的关键，它们起着运输放大细胞的作用。最近，在体外扩增啮齿类动物的生殖系干细胞已成为可能。此外，从新生小鼠睾丸中已经建立了功能上与胚胎干细胞相同的多能干细胞。这些来自睾丸的干细胞应该有助于生物学研究和技术。另一方面，由于缺乏合适的实验系统，人类精子发生的本质在很大程度上是未知的。然而，流行的睾丸精子提取程序揭开了人类精子发生的未知方面。人精原干细胞培养方法的建立，有望在不久的将来，将是一个很大的好处，实现进一步了解人类精子发生，并应导致更复杂的诊断和治疗男性不育症的临床措施。
Abstract: Germ cells are defined by their innate potential to transmit genetic information to the next generation through fertilization. Males produce numerous sperm for long periods to maximize chances of fertilization. Key to the continuous production of large numbers of sperm are germline stem cells and their immediate daughter cells, functioning as transit amplifying cells. Recently, it has become possible to expand germline stem cells of rodents in vitro. In addition, multipotent stem cells, which are functionally the same as embryonic stem cells, have been established from neonatal mouse testes. These stem cells derived from the testis should contribute to biological research and technologies. On the other hand, the nature of human spermatogenesis is largely unknown due to the lack of an appropriate experimental system. However, the prevailing testicular sperm extraction procedure unraveled hitherto unknown facets of human spermatogenesis. The establishment of a culturing method for human spermatogonial stem cells in hopefully the near future would be a great benefit for achieving further insight into human spermatogenesis and should lead to more sophisticated diagnostic and therapeutic clinical measures for male infertility.