Quantifying the C-reactive protein concentrations of uterine lavage samples in postpartum dairy cows
Quantifying the C-reactive protein concentrations of uterine lavage samples in postpartum dairy cows
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DOI：
10.1016/j.anireprosci.2020.106455
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发表时间：
2020-06-01
影响因子：
2.2
通讯作者：
Tanaka, Tomomi
中科院分区：
文献类型：
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作者：
Tanai, Shunsuke;Endo, Natsumi;Tanaka, Tomomi
研究了分娩后奶牛子宫灌洗液中C反应蛋白(CRP)浓度的变化及其与细胞学结果的关系。8头荷斯坦奶牛分别于产后3周和6周进行子宫冲洗，测定中性粒细胞(PMN：ALL)和淋巴细胞(PMN：LYM)比值和C反应蛋白(CRP)浓度。采集血液样本以监测代谢变量，并使用在子宫灌洗发生的同时收集的样本来定量血浆CRP浓度。细胞学检查确定PMN：ALL和PMN：LYM。在整个产后期间，代谢变量的值在正常范围内。PMN：ALL与PMN：LYM之间存在相关性，产后6周时两者比值均小于3周(P<；0.05)。产后6周子宫灌洗液中C反应蛋白平均浓度(27.7±9.6 ng/ml)低于产后3周(60.8±28.1 ng/ml)，而血浆C反应蛋白浓度在产后6周(287.4±34.1 ng/ml)高于产后3周(254.8±29.4 ng/ml)。本研究结果表明，在子宫退缩过程中，子宫灌洗液中的CRP浓度与PMN的出现频率呈平行的下降趋势，提示子宫灌洗液中的CRP浓度可作为评价奶牛子宫炎症的局部生物标志物。
Changes in the C-reactive protein (CRP) concentration, a non-specific diagnostic biomarker, in uterine lavage fluid and associations with cytological findings were examined following parturition in dairy cows. In postpartum Holstein dairy cows (n = 8), uterine lavage was performed at 3 and 6 weeks postpartum, and polymorphonuclear leukocyte (PMN) ratios to total cells (PMN:ALL) and to lymphocytes (PMN:LYM) and CRP concentrations were determined. Blood samples were collected to monitor the metabolic variables, and plasma CRP concentrations were quantified using samples collected at the same time as the uterine lavage occurred. A cytological examination was performed to determine PMN:ALL and PMN:LYM. The values for metabolic variables were within the normal range throughout the postpartum period. There was a correlation between the PMN:ALL and PMN:LYM, and both ratios were less (P < 0.05) at 6 than 3 weeks postpartum. The mean CRP concentration of the uterine lavage fluid was less at 6 (27.7 +/- 9.6 ng/ml) than 3 weeks (60.8 +/- 28.1 ng/ml) postpartum, whereas mean plasma CRP concentration was greater at 6 (287.4 +/- 34.1 ng/ml) than 3 (254.8 +/- 29.4 ng/ml) weeks postpartum. The results of the present study indicate the CRP concentration of uterine lavage fluid decreased in parallel with the frequency of PMN during uterine involution, which leads to the suggestion that uterine lavage fluid CRP concentration could be utilized as a local biomarker for evaluating uterine inflammation in cows.