Relaxin increases secretion of tissue inhibitor of matrix metalloproteinase-1 and-2 during uterine and cervical growth and remodeling in the pig
Relaxin increases secretion of tissue inhibitor of matrix metalloproteinase-1 and-2 during uterine and cervical growth and remodeling in the pig
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DOI：
10.1210/en.143.1.91
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发表时间：
2002-01-01
期刊：
影响因子：
4.8
通讯作者：
Bagnell, CA
中科院分区：
文献类型：
--
作者：
Lenhart, JA;Ryan, PL;Bagnell, CA
怀孕期间生殖器官的重塑需要降解和重新合成细胞入侵的结构障碍。基质金属蛋白酶 (MMP) 是分解细胞外基质 (ECM) 成分的酶，对于组织重塑过程至关重要。金属蛋白酶组织抑制剂 (TIMP) 是 MMP 活性的重要调节剂。在猪中，松弛素在怀孕期间刺激子宫和子宫颈的生长和重塑，其影响包括改变 ECM 元素的能力。因此，本研究的目的是确定松弛素是否改变猪子宫或子宫颈中 TIMP-1 和 TIMP-2 的产生和/或活性。松弛素的生长促进作用是通过每 6 小时向青春期前的小母猪施用松弛素，持续 54 小时而产生的。通过免疫印迹来表征 TIMP-1 和 TIMP-2 的表达。使用 MMP 特异性荧光底物测定法测量总酶活性。 TIMP-1 和 TIMP-2 蛋白存在于对照的子宫和子宫颈中，而松弛蛋白则存在于子宫冲洗和组织中（P
Remodeling of reproductive organs during pregnancy requires degradation and resynthesis of structural barriers to cell invasion. Matrix metalloproteinases (MMPs) are enzymes that break down components of the extracellular matrix (ECM) and are essential for tissue remodeling processes. Tissue inhibitors of metalloproteinases (TIMPs) are important regulators of MMP activity. In the pig, relaxin stimulates growth and remodeling of the uterus and cervix during pregnancy, effects that include the ability to alter elements of the ECM. Therefore, the objective of this study was to determine whether relaxin alters the production and/or activity of TIMP-1 and TIMP-2 in the porcine uterus or cervix. The growth-promoting effects of relaxin were elicited by administering relaxin to prepubertal gilts every 6 h for 54 h. Expression of TIMP-1 and TIMP-2 was characterized by immunoblotting. Total enzyme activity was measured using an MMP-specific fluorescent substrate assay. TIMP-1 and TIMP-2 proteins were present in the uterus and cervix of control and laxin in the uterine flushes and tissues (P