Wavefront corrected light sheet microscopy in turbid media
Wavefront corrected light sheet microscopy in turbid media
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DOI：
10.1063/1.4710527
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发表时间：
2012-05-07
影响因子：
4
通讯作者：
Dholakia, K.
中科院分区：
文献类型：
--
作者：
Dalgarno, H. I. C.;Cizmar, T.;Dholakia, K.
光片显微镜是一种对大型生物标本进行三维成像的有效方法。然而，由于样品的散射和像差的影响，其成像能力大大降低。光学清除、贝塞尔光模和背景抑制已经被用来试图避开这些有害的影响。我们提出了一种原位波前校正，通过在混浊的样品中创建一个“最佳”的光片来提供一个重大的进步。重要的是，我们表明，不需要组织清理或专门的样品制备，并且在基于高斯和贝塞尔光束的光片模式下，图像质量和深度分辨率都得到了明显的改善。(C)2012年美国物理研究所。[http://dx.doi.org/10.1063/1.4710527]
Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an "optimal" light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4710527]