Tumour infiltrating lymphocytes and apoptosis are independent features in colorectal cancer stratified according to microsatellite instability status
Tumour infiltrating lymphocytes and apoptosis are independent features in colorectal cancer stratified according to microsatellite instability status
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DOI：
10.1136/gut.48.3.360
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发表时间：
2001-03-01
期刊：
影响因子：
24.5
通讯作者：
Radford-Smith, GL
中科院分区：
文献类型：
--
作者：
Michael-Robinson, JM;Biemer-Hüttmann, AE;Radford-Smith, GL
背景：结直肠癌中存在高水平的DNA微卫星不稳定性（MSI-H）与预后改善有关，肿瘤浸润淋巴细胞（TILs）的存在也是如此。目前尚不清楚til是否通过激活抗肿瘤免疫反应直接促进与MSI-H癌症相关的生存优势。目的：探讨MSI分层结直肠癌TIL与细胞凋亡的相关性。方法：在选定的102例散发性结直肠癌中，根据MSI水平分为32例MSI- h， 30例MSI-低（MSI- l）和40例微卫星稳定（MSS），对TILs的分布进行了表征和量化。使用T细胞标记CD3和CD8以及B细胞标记CD20对档案块进行免疫染色。免疫组化M30 CytoDEATH抗体定量检测恶性上皮细胞凋亡。结果：抗cd3阳性染色和抗cd20阴性染色表明，几乎所有TILs都是T细胞。大多数CD3(+) TILs（约75%）也被抗cds染色。TIL在MSI-H结直肠癌中最为丰富，其中23/32（72%）为TIL阳性。只有5/40（12.5%）的MSS肿瘤和9/30（30%）的MSI-L肿瘤TIL阳性(p
Background-The presence of high level DNA microsatellite instability (MSI-H) in colorectal cancer is associated with an improved prognosis, as is the presence of tumour infiltrating lymphocytes (TILs). It is not clear if TILs contribute directly to the survival advantage associated with MSI-H cancers through activation of an antitumour immune response.Aims-To correlate TIL and apoptosis rates in colorectal cancer stratified by MSI status.Methods-The distribution of TILs was characterised and quantified in a selected series of 102 sporadic colorectal cancers classified according to levels of MSI as 32 MSI-H, 30 MSI-low (MSI-L), and 40 microsatellite stable (MSS). Archival blocks were immunostained using the T cell markers CD3 and CD8, and the B cell marker CD20. Apoptosis of malignant epithelial cells was quantified by immunohistochemistry with the M30 CytoDEATH antibody.Results-Positive staining with anti-CD3 and negative staining with anti-CD20 identified virtually all TILs as T cells. The majority of CD3(+) TILs (>75%) also stained with anti-CDS. TILs were most abundant in MSI-H colorectal cancers in which 23/32 (72%) scored as TIL positive. Only 5/40 (12.5%) MSS tumours and 9/30 (30%) MSI-L cancers were TIL positive (p