Interaction between a negative regulator (Msa2/Nrd1) and a positive regulator (Cpc2) of sexual differentiation in Schizosaccharomyces pombe
Interaction between a negative regulator (Msa2/Nrd1) and a positive regulator (Cpc2) of sexual differentiation in Schizosaccharomyces pombe
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DOI：
10.1271/bbb.68.1621
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发表时间：
2004-07-01
影响因子：
1.6
通讯作者：
Kawamukai, M
中科院分区：
文献类型：
--
作者：
Jeong, HT;Oowatari, Y;Kawamukai, M
裂殖酵母的性别分化是由许多尚未完全确定的细胞成分控制的。我们分离到一个称为msa2的基因，作为一个产孢子异常突变体(Sam1)的多拷贝抑制基因。Msa2p与Nrd1p相同，Nrd1p被认为是阻止性别分化开始的一个因素。利用酵母双杂交系统鉴定与Msa2p/Nrd1p相互作用的RACK1蛋白的裂解酵母同源物Cpc2p。我们证实Msa2p/Nrd1p在S.pombe细胞中与Cpc2p相互作用。对msa2/nrd1和cpc2的上位性分析表明，msa2p/Nrd1p是cpc2p的上游调控因子。对Cpc2p和Msa2p/Nrd1p的定位分析表明，这两种蛋白主要定位在细胞质中。负性调控因子Msa2p/Nrd1p与正性调控因子Cpc2p的相互作用提示了一种新的调控机制。
The sexual differentiation of Schizosaccharomyces pombe is controlled by many cellular components which have not been fully characterized. We isolated a gene called msa2 as a multi-copy suppressor of a sporulation abnormal mutant (sam1). Msa2p is identical with Nrd1p which has been characterized as a factor that blocks the onset of sexual differentiation. The yeast two-hybrid system was used to identify Cpc2p, a fission yeast homolog of the RACK1 protein, that interacted with Msa2p/Nrd1p. We confirmed that Msa2p/Nrd1p interacted with Cpc2p in S. pombe cells. An epistatic analysis of msa2/nrd1 and cpc2 suggests that Msa2p/Nrd1p was an upstream regulator for Cpc2p. A localization analysis of Cpc2p and Msa2p/Nrd1p indicates that both proteins were predominantly localized in the cytoplasm. The interaction of negative regulator Msa2p/Nrd1p with positive regulator Cpc2p suggests a new regulatory circuit in the sexual differentiation of S. pombe.