Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (Bombyx mori) muscle contraction assay
Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (Bombyx mori) muscle contraction assay
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DOI：
10.5582/ddt.2012.v6.2.88
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发表时间：
2012-04-01
影响因子：
3.1
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
--
作者：
Fujiyuki, Tomoko;Hamamoto, Hiroshi;Sekimizu, Kazuhisa
在家蚕幼虫中，麻痹肽（PP）（一种昆虫细胞因子）的成熟形式由PP原产生，其与先天免疫应答的激活相关，导致缓慢的肌肉收缩。我们利用这种反应，在家蚕肌肉收缩加上先天免疫刺激，定量测量各种天然多糖的先天免疫刺激活性。Gyrophora esculenta的β-葡聚糖（GE-3）、裙带菜孢子叶中的岩藻依聚糖和curldan诱导家蚕肌肉收缩。进一步证明了GE-3对家蚕杆状病毒感染具有治疗作用。基于这些发现，我们提出，家蚕肌肉收缩试验是有用的筛选物质，刺激先天免疫之前，评估治疗效果的哺乳动物。
In silkworm larvae, the mature form of paralytic peptide (PP), an insect cytokine, is produced from pro-PP in association with activation of innate immune responses, resulting in slow muscle contraction. We utilized this reaction, muscle contraction in silkworms coupled with innate immunity stimulation, to quantitatively measure the innate immune stimulating activity of various natural polysaccharides. beta-Glucan of Gyrophora esculenta (GE-3), fucoidan from sporophyll of Undaria pinnatifida, and curldan induced silkworm muscle contraction. We further demonstrated that GE-3 had therapeutic effects on silkworms infected by baculovirus. Based on these findings, we propose that the silkworm muscle contraction assay is useful for screening substances that stimulate innate immunity before evaluating therapeutic effectiveness in mammals.