Determination of organophosphate pesticides at a carbon nanotube/organophosphorus hydrolase electrochemical biosensor
Determination of organophosphate pesticides at a carbon nanotube/organophosphorus hydrolase electrochemical biosensor
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DOI：
10.1016/j.aca.2004.09.072
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发表时间：
2005-02-14
影响因子：
6.2
通讯作者：
Lin, YH
中科院分区：
文献类型：
--
作者：
Deo, RP;Wang, J;Lin, YH
报道了一种基于碳纳米管（CNT）修饰的传感器和有机磷水解酶（OPH）生物催化剂的有机磷农药电流型生物传感器。一个双层的方法与OPH层顶部的CNT膜用于制备CNT/OPH生物传感器。CNT层导致酶促产生的对硝基苯酚产物的阳极检测大大改善，包括更高的灵敏度和稳定性。传感器的性能进行了优化的表面改性和操作条件。在最佳条件下，该生物传感器用于测量低至0.15 μ M的对氧磷和0.8 μ M的甲基对硫磷，灵敏度分别为25和6 nA/μ LM。(C)2004 Elsevier B. V.保留所有权利。
An amperometric biosensor for oganophosphorus (OP) pesticides based on a carbon nanotube (CNT)-modified transducer and an organophosphorus hydrolase (OPH) biocatalyst is described. A bilayer approach with the OPH layer atop of the CNT film was used for preparing the CNT/OPH biosensor. The CNT layer leads to a greatly improved anodic detection of the enzymatically generated p-nitrophenol product, including higher sensitivity and stability. The sensor performance was optimized with respect to the surface modification and operating conditions. Under the optimal conditions the biosensor was used to measure as low as 0.15 muM paraoxon and 0.8 muM methyl parathion with sensitivities of 25 and 6 nA/muLM, respectively. (C) 2004 Elsevier B.V. All rights reserved.