Human Endogenous Retrovirus (HERV)-R family in primates: Chromosomal location, gene expression, and evolution
Human Endogenous Retrovirus (HERV)-R family in primates: Chromosomal location, gene expression, and evolution
复制标题
DOI：
10.1016/j.gene.2005.11.008
复制
发表时间：
2006-03-29
期刊：
影响因子：
3.5
通讯作者：
Lee, WH
中科院分区：
文献类型：
--
作者：
Kim, HS;Yi, JM;Lee, WH
到目前为止，全长内源性逆转录病毒(Herv)-R已定位于人类染色体7q11.2，并在胎盘和其他类型的细胞中检测到信使核糖核酸和包膜蛋白。在本研究中，我们利用来自大猩猩融合体库的探针，检测了人类染色体7q11.2中Herv-Rem基因的同源基因(7q31.3)，并确定了其在猿猴和东半球猴中的染色体位置。通过FISH和PCR分析，在新大陆猴子或原猴身上没有检测到Herv-R基因。我们用聚合酶链式反应和测序工具测定了从灵长类动物基因组DNA中获得的HERV-R env基因的序列。除了来自大猩猩DNA的Herv-R env序列外，推测的包膜蛋白的功能结构域是保守的，这表明这些结构域可能在灵长类基因组中具有功能能力。此外，我们还研究了HERV-R在不同组织和癌细胞中的ENT1基因表达。RT-PCR方法表明，em1基因在几种人体组织(脑、前列腺、睾丸、肾、胎盘、胸腺和子宫)和癌细胞(RT4、BT-474、MCF7、OVCAR-3、LOX-IMVI和AZ521)中都有表达。综上所述，我们的数据可能对理解HERV-R通过灵长类辐射的进化动力学以及它在人类组织和癌细胞中的功能作用的含义有很大帮助。(C)2005 Elsevier B.V.保留所有权利。
Hitherto, full-length endogenous retrovirus (HERV)-R has been located at human chromosome 7q11.2, and mRNA and envelope proteins have been detected in placenta and a variety of other cell types. In the present study, using a probe derived from the gorilla fosmid library, we detected the paralogous locus (7q31.3) of the HERV-R em, gene in human chromosome 7q11.2, and also determined the chromosomal location in apes and Old World monkeys. The HERV-R gene was not detected in New World monkeys or prosimians with FISH and PCR analyses. We determined the sequences of the HERV-R env genes obtained from the genomic DNA of primates using PCR and sequencing tools. Except for a HERV-R env, sequence derived from gorilla DNA, the functional domains of putative envelope proteins are conserved, suggesting that those domains could have a functional capacity in the primate genome. In addition, we investigated the ent, gene expression of HERV-R in various human tissues and cancer cells. An RT-PCR approach indicated that the em, gene was expressed in several human tissues (brain, prostate, testis, kidney, placenta, thymus, and uterus) and cancer cells (RT4, BT-474, MCF7, OVCAR-3, LOX-IMVI, and AZ521). Taken together, our data could be of Great use for understanding the evolutionary dynamics of HERV-R through primate radiation as well as the implications of its functional role in human tissues and cancers cells. (c) 2005 Elsevier B.V. All rights reserved.