Stability improvement and characterization of bioprinted pectin-based scaffold
Stability improvement and characterization of bioprinted pectin-based scaffold
复制标题
生物打印果胶支架的稳定性改进和表征
DOI：
10.1177/2280800018807108
复制
发表时间：
2019-02-01
影响因子：
2.5
通讯作者：
Zhang, Wujie
中科院分区：
文献类型：
--
作者：
Stealey, Samuel;Guo, Xiaoru;Zhang, Wujie
目的：生物打印是构建用于移植的组织/器官的替代方法。本研究探讨了交联剂的影响和印刷后的修改，使用低聚壳聚糖和壳聚糖的稳定性改善。方法：以壳寡糖为交联剂，取代钙离子制备果胶基生物墨水。采用低聚壳聚糖（2kD）和不同分子量的壳聚糖对生物打印支架进行改性。采用傅里叶变换红外光谱（FTIR）和扫描电镜（SEM）对支架进行表征。结果：壳寡糖不能作为一种可行的交联剂。通过FTIR和SEM分析证实了成功的印刷后改性。结论：对于后修饰，壳聚糖处理的支架显示出增强的稳定性相比，未经处理的支架。特别是，用150 kD壳聚糖修饰的支架表现出最高的稳定性。
Purpose: Bioprinting is an alternative method for constructing tissues/organs for transplantation. This study investigated the cross-linker influence and post-printing modification using oligochitosan and chitosan for stability improvement. Methods: Oligochitosan was tested as a novel cross-linker to replace Ca2+ for pectin-based bio-ink. Oligochitosan (2 kD) and different molecular weight of chitosan were used to modify the bioprinted scaffold. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to characterize the scaffolds. Results: Oligochitosan failed to serve as a viable cross-linker. Successful post-printing modification was confirmed by FTIR and SEM analyses. Conclusion: Regarding post-modification, chitosan-treated scaffolds showed enhanced stability compared to untreated scaffolds. In particular, scaffolds modified with 150 kD chitosan exhibited the highest stability.