Transgenerational epigenetic programming of the embryonic testis transcriptome
Transgenerational epigenetic programming of the embryonic testis transcriptome
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DOI：
10.1016/j.ygeno.2007.10.002
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发表时间：
2008-01-01
期刊：
影响因子：
4.4
通讯作者：
Skinner, Michael K.
中科院分区：
文献类型：
--
作者：
Anway, Matthew D.;Rekow, Stephen S.;Skinner, Michael K.
胚胎在性腺性别决定期间暴露于内分泌干扰物长春花碱，似乎促进了男性生殖系的表观遗传重新编程，这与跨代成人发病疾病状态有关。胚胎第16天(E16)睾丸的跨代效应表明，睾丸转录组在多个世代(F1-F3)中发生了可重复的变化。发现196个基因的表达受到影响，大部分基因表达下调或沉默。在F1代和F2代长春花碱(E16)胚胎睾丸中，甲基转移酶基因的表达在性腺性别决定过程中发生了显著的变化，但到F3代时大部分恢复到对照世代水平。最显著的影响发生在与生殖系相关的DNMT3A和DNMT3L亚型上。观察表明，胚胎暴露于长春新灵似乎促进了男性生殖系的表观遗传学重新编程，这与随后几代人睾丸转录组的跨代变化有关。由爱思唯尔公司出版。
Embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination appears to promote an epigenetic reprogramming of the male germ line that is associated with transgenerational adult-onset disease states. Transgenerational effects on the embryonic day 16 (E16) testis demonstrated reproducible changes in the testis transcriptome for multiple generations (F1-F3). The expression of 196 genes was found to be influenced, with the majority of gene expression being decreased or silenced. Dramatic changes in the gene expression of methyltransferases during gonadal sex determination were observed in the F1 and F2 vinclozolin generation (E16) embryonic testis, but the majority returned to control-generation levels by the F3 generation. The most dramatic effects were on the germ-line-associated Dnmt3A and Dnmt3L isoforms. Observations demonstrate that an embryonic exposure to vinclozolin appears to promote an epigenetic reprogramming of the male germ line that correlates with transgenerational alterations in the testis transcriptome in subsequent generations. Published by Elsevier Inc.