Vein wall remodeling after deep vein thrombosis involves matrix metalloproteinases and late fibrosis in a mouse model
Vein wall remodeling after deep vein thrombosis involves matrix metalloproteinases and late fibrosis in a mouse model
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DOI：
10.1016/j.jvs.2005.04.014
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发表时间：
2005-07-01
影响因子：
4.3
通讯作者：
Henke, PK
中科院分区：
文献类型：
--
作者：
Deatrick, KB;Eliason, JL;Henke, PK
假说.深静脉血栓形成（DVT）导致静脉壁损伤，与纤维化和细胞外基质（ECM）周转相关，可能由基质蛋白酶介导。本研究探讨了蛋白酶和胶原蛋白在早期静脉壁重塑中的表达。在小鼠中，通过结扎肾下下腔静脉来产生DVT。下腔静脉（IVC）或假手术，并在第4、8和12天收获组织。处理静脉壁组织进行实时逆转录酶-聚合酶链反应（每个时间点6 - 8个）、Western免疫印迹（每个时间点5个）和明胶酶谱（每个时间点5个）。采用方差分析进行多重比较，P
Hypothesis. Deep venous thrombosis (DVT) confers vein wall injury associated with fibrosis and extracellular matrix (ECM) turnover, likely mediated by matrix proteases. This study investigated the expression of proteases and collagen involved in early vein wall remodeling.Methods. In the mouse, DVT was produced by ligation of the infrarenal inferior vena. cava (IVC) or sham operation, and tissue was harvested at 4, 8, and 12 days. The vein wall tissue was processed for real-time reverse transcriptase-polymerase chain reaction (6 to 8 per time point), Western immunoblotting (5 per time point), and gelatin zymography (5 per time point). Analysis of variance was used for multiple comparisons, and a P