UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides
UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides
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DOI：
10.1016/j.febslet.2012.03.003
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发表时间：
2012-04-05
期刊：
影响因子：
3.5
通讯作者：
Mizukami, Hajime
中科院分区：
文献类型：
--
作者：
Nagatoshi, Mai;Terasaka, Kazuyoshi;Mizukami, Hajime
藏红花素是栀子果实中积累的一种脱辅基类胡萝卜素糖基酯，用作食用色素和营养品。首次在G.已经鉴定并功能性地表征了Jasminoides。UGT 75 L6优先使藏红花酸的羧基葡糖基化，产生藏红花酸葡糖基酯，而UGT 94 E5使藏红花酸葡糖基酯的葡萄糖部分的60位羟基葡糖基化。UGT 75 L6和UGT 94 E5的表达模式与G.茉莉花(C)2012年欧洲生物化学学会联合会。由Elsevier B出版。版权所有© 2016
Crocin is an apocarotenoid glycosyl ester accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical. For the first time, the two glucosyltransferases UGT75L6 and UGT94E5 that sequentially mediate the final glucosylation steps in crocin biosynthesis in G. jasminoides have been identified and functionally characterized. UGT75L6 preferentially glucosylates the carboxyl group of crocetin yielding crocetin glucosyl esters, while UGT94E5 glucosylates the 60 hydroxyl group of the glucose moiety of crocetin glucosyl esters. The expression pattern of neither UGT75L6 nor UGT94E5 correlated with the pattern of crocin accumulation in G. jasminoides. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.