Diosgenin present in fenugreek improves glucose metabolism by promoting adipocyte differentiation and inhibiting inflammation in adipose tissues.
Diosgenin present in fenugreek improves glucose metabolism by promoting adipocyte differentiation and inhibiting inflammation in adipose tissues.
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DOI：
10.1002/mnfr.200900609
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发表时间：
2010-11
影响因子：
5.2
通讯作者：
Taku Uemura;Shizuka Hirai;N. Mizoguchi;T. Goto;Joo-Yong Lee;K. Taketani;Yuki Nakano;Jinji Shono;Shohei Hoshino;N. Tsuge;Toshihiko Narukami;N. Takahashi;T. Kawada
中科院分区：
文献类型：
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作者：
Taku Uemura;Shizuka Hirai;N. Mizoguchi;T. Goto;Joo-Yong Lee;K. Taketani;Yuki Nakano;Jinji Shono;Shohei Hoshino;N. Tsuge;Toshihiko Narukami;N. Takahashi;T. Kawada
在肥胖症中，脂肪细胞肥大和脂肪组织中的慢性炎症会导致胰岛素抵抗和2型糖尿病。葫芦巴（胡芦巴）可以改善高血糖和糖尿病。然而，胡芦巴对脂肪细胞大小和脂肪组织炎症的影响尚未得到证实。在这项研究中，我们确定了胡芦巴对糖尿病肥胖 KK-Ay 小鼠脂肪细胞大小和脂肪组织炎症的影响，并鉴定了胡芦巴中的活性物质。用添加 2% 胡芦巴的高脂肪饮食治疗 KK-Ay 小鼠可改善糖尿病。此外，胡芦巴使脂肪细胞微型化，并增加脂肪组织中分化相关基因的 mRNA 表达水平。胡芦巴还抑制巨噬细胞浸润脂肪组织并降低炎症基因的 mRNA 表达水平。此外，我们还发现了薯蓣皂苷元，胡芦巴中皂苷的主要苷元，可促进脂肪细胞分化并抑制与 3T3-L1 细胞炎症相关的几种候选分子的表达。这些结果表明胡芦巴通过促进脂肪细胞分化和抑制脂肪组织炎症来改善糖尿病，其作用是由薯蓣皂苷元介导的。含有薯蓣皂苷元的胡芦巴可用于改善与肥胖相关的葡萄糖代谢紊乱。
In obesity, adipocyte hypertrophy and chronic inflammation in adipose tissues cause insulin resistance and type-2 diabetes. Trigonella foenum-graecum (fenugreek) can ameliorate hyperglycemia and diabetes. However, the effects of fenugreek on adipocyte size and inflammation in adipose tissues have not been demonstrated. In this study, we determined the effects of fenugreek on adipocyte size and inflammation in adipose tissues in diabetic obese KK-Ay mice, and identified the active substance in fenugreek. Treatment of KK-Ay mice with a high fat diet supplemented with 2% fenugreek ameliorated diabetes. Moreover, fenugreek miniaturized the adipocytes and increased the mRNA expression levels of differentiation-related genes in adipose tissues. Fenugreek also inhibited macrophage infiltration into adipose tissues and decreased the mRNA expression levels of inflammatory genes. In addition, we identified diosgenin, a major aglycone of saponins in fenugreek to promote adipocyte differentiation and to inhibit expressions of several molecular candidates associated with inflammation in 3T3-L1 cells. These results suggest that fenugreek ameliorated diabetes by promoting adipocyte differentiation and inhibiting inflammation in adipose tissues, and its effects are mediated by diosgenin. Fenugreek containing diosgenin may be useful for ameliorating the glucose metabolic disorder associated with obesity.