Reference values for peripheral blood B-lymphocyte subpopulations:: a basis for multiparametric immunophenotyping of abnormal lymphocytes
Reference values for peripheral blood B-lymphocyte subpopulations:: a basis for multiparametric immunophenotyping of abnormal lymphocytes
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DOI：
10.1016/s0022-1759(01)00338-6
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发表时间：
2001-07-01
影响因子：
2.2
通讯作者：
De Bruyère, M
中科院分区：
文献类型：
--
作者：
Deneys, V;Mazzon, AM;De Bruyère, M
背景和目的：免疫表型分析已成为鉴别诊断慢性B细胞淋巴增生性疾病的有用工具。这项工作的目的是确定正常B细胞亚群的参考值。材料和方法：使用一组直接缀合的抗体，通过多维流式细胞术分析来自38名健康志愿者的血液样品。结果表示为阳性B细胞的百分比和中值荧光强度，表达水平的间接评估。结果如下：CD 20、CD 22、CD 24、CD 40、CD 79 a、CD 79 B b、FMC 7、CD 11 a、CD 18、CD 44在整个B细胞群中呈阳性表达，而CD 10、CD 86、CD 103、CD 154和Fast在B淋巴细胞群中几乎不表达。IgD阳性率为75%。kappa/lambda比值为1.5。CD 5、CD 23、CD 25、CD 38、CD 43、CD 54、CD 62 L、CD 80和CD 95在不同的B细胞亚群中呈阳性表达。所有这些标志物在慢性B细胞淋巴增生性疾病的鉴别诊断的效用进行了讨论。结论：为了解释病理免疫表型，有必要参考正常B细胞亚群的定量和定性值。(C)2001 Elsevier Science B. V.保留所有权利。
Background and objectives: Immunophenotyping has become a useful tool for the differential diagnosis of chronic B-cell lymphoproliferative disorders. The aim of this work was to determine reference values of normal B-cell subpopulations. Material and methods: Blood samples from 38 healthy volunteers were analyzed by multidimensional flow cytometry, using a panel of directly conjugated antibodies. Results were expressed as percent of positive B cells and as median fluorescence intensity, an indirect assessment of the expression level. Results: CD20, CD22, CD24, CD40, CD79a, CD79b, FMC7, CD11a, CD18, CD44 were positive in the whole B cell population, whereas CD10, CD86, CD103, CD154 and Fast were almost absent from the B-lymphocyte population. 75% were IgD positive. The kappa/lambda ratio was 1.5. CD5, CD23, CD25, CD38, CD43, CD54, CD62L, CD80 and CD95 were positive in different B-cell subpopulations. The utility of all these markers in the differential diagnosis of chronic B-cell lymphoproliferative disorders is discussed. Conclusion: In order to interpret a pathological immunophenotype, it is necessary to refer to quantitative and qualitative values of normal B-cell subpopulations. (C) 2001 Elsevier Science B.V. All rights reserved.