KINETICS AND INHIBITION BY SOME AMINO-ACIDS OF LACTATING RAT MAMMARY-GLAND ARGINASE
KINETICS AND INHIBITION BY SOME AMINO-ACIDS OF LACTATING RAT MAMMARY-GLAND ARGINASE
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DOI：
10.3109/13813459409003940
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发表时间：
1994-09-01
期刊：
影响因子：
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通讯作者：
SOLER, G
中科院分区：
文献类型：
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作者：
FUENTES, JM;CAMPO, ML;SOLER, G
本文研究了哺乳期大鼠乳腺过氧化物酶的某些动力学和调节特性。在pH 7.4，即在接近生理条件下，存在过量底物抑制的证据，K-m值为9.5 mM，略低于在pH 9.8（最大酶活性）下观察到的18 mM值。还研究了脯氨酸、鸟氨酸、赖氨酸和某些支链氨基酸对酶活性的影响：赖氨酸、鸟氨酸和缬氨酸强烈竞争性抑制泌乳大鼠乳腺脱氢酶，Ki值分别为1.2 mM、1.1 mM和3.6 mM。其他氨基酸（脯氨酸，异亮氨酸和亮氨酸）也抑制泌乳大鼠乳腺脱氢酶，虽然在较小程度上。
Some kinetic and regulatory properties of lactating rat mammary gland arginase were studied. At pH 7.4, i.e. at near-physiological conditions, there was evidence of inhibition by excess of substrate, with a K-m value of 9.5 mM, slightly lower than the value of 18 mM observed at pH 9.8 (maximum enzyme activity). A study was also made of the effects of proline, ornithine, lysine and certain branched-chain aminoacids on enzyme activity : lactating rat mammary gland arginase was strongly and competitively inhibited by lysine, ornithine and valine, with Ki values of 1.2 mM, 1.1 mM and 3.6 mM, respectively. Other aminoacids (proline, isoleucine and leucine) also inhibited lactating rat mammary gland arginase, although to a lesser extent.