Characterization of nineteen microsatellite markers and development of multiplex PCRs for the wedge clam Donax trunculus (Mollusca: Bivalvia)
Characterization of nineteen microsatellite markers and development of multiplex PCRs for the wedge clam Donax trunculus (Mollusca: Bivalvia)
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DOI：
10.1007/s11033-014-3406-0
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发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Freire, Ruth
中科院分区：
文献类型：
--
作者：
Nanton, Ana;Arias-Perez, Alberto;Freire, Ruth
楔蛤Donax trunculus是一种南极-地中海暖温带物种，分布于塞内加尔至法国北方海岸，包括地中海和黑海。它在几个欧洲国家进行商业开发，由于其高经济价值而成为重要的渔业资源。为了有助于其保护和管理，从两个富集的基因组文库中分离出19个微卫星标记。这些位点的特点是在30蛤从一个单一的人口从西班牙西北部。每个位点的等位基因数为2 ~ 17个，观察杂合度和期望杂合度分别为0 ~ 0.714和0.078 ~ 0.950。未检测到连锁不平衡，9个位点符合Hardy-Weinberg平衡。15个多态性标记被安排到三个多重PCR组中，以减少微卫星基因分型的时间和成本。这是首次报道的多态性微卫星标记。躯干这些新的标记为今后的种群遗传学研究和该物种的管理和养殖提供了宝贵的资源。
The wedge clam Donax trunculus is an Atlantic-Mediterranean warm-temperate species found from Senegal to the northern coast of France, including the Mediterranean and Black Sea. It is commercially exploited in several European countries and constitutes an important fishing resource due to its high economical value. To contribute to its conservation and management, nineteen microsatellite markers were isolated from two enriched genomic libraries. These loci were characterized in 30 clams from a single population from northwest Spain. The number of alleles per locus ranged from 2 to 17 and observed and expected heterozygosity varied from zero to 0.714 and from 0.078 to 0.950, respectively. Linkage disequilibrium was not detected and nine loci were in agreement with Hardy-Weinberg equilibrium. Fifteen polymorphic markers were arranged into three multiplex PCR sets to reduce both time and cost of microsatellite genotyping. This is the first time that polymorphic microsatellite markers have been reported for D. trunculus. These new markers provide a valuable resource for future population genetics studies and management and culture of this species.