Characteristics of central binding sites for [3H] DAMGO in spontaneously hypertensive rats.
Characteristics of central binding sites for [3H] DAMGO in spontaneously hypertensive rats.
复制标题
自发性高血压大鼠中 [3H] DAMGO 中心结合位点的特征。
DOI：
10.1016/0024-3205(90)90229-k
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发表时间：
1990
期刊：
影响因子：
6.1
通讯作者：
Bhargava,HN
中科院分区：
文献类型：
--
作者：
Gulati,A;Bhargava,HN
研究了10周龄自发性高血压（SHR）和正常Wistar-Kyoto （WKY）大鼠脑离散区和脊髓膜与μ-阿片受体高选择性配体[3H] DAMGO的结合情况。检查的脑区为下丘脑、杏仁核、海马、纹状体、脑桥和髓质、中脑和皮层。[3H] DAMGO在一个单一的高亲和力位点结合到脑区和脊髓的膜上。[3H] DAMGO结合WKY和SHR大鼠海马、纹状体、脑桥和髓质、皮质和脊髓膜的受体密度（Bmaxvalue）和表观解离常数（Kdvalue）无显著差异。SHR大鼠下丘脑和中脑膜中[3H] DAMGO的bmax值显著高于WKY大鼠，但kdvalue在两株大鼠中无显著差异。另一方面，SHR大鼠杏仁核膜中[3H] DAMGO的bmax值低于WKY大鼠，但两者的kd值相似。由此可见，与WKY大鼠相比，SHR大鼠下丘脑和中脑的μ-阿片受体密度较高，杏仁核的μ-阿片受体密度较低，这种分布差异可能与SHR大鼠血压升高有关。
The binding of [3H] DAMGO, a highly selective ligand for μ-opiate receptors, to membranes of discrete brain regions and spinal cord of 10 week old spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats was determined. The brain regions examined were hypothalamus, amygdala, hippocampus, corpus striatum, pons and medulla, midbrain and cortex. [3H] DAMGO bound to membranes of brain regions and spinal cord at a single high affinity site. The receptor density (Bmaxvalue) and apparent dissociation constant (Kdvalue) of [3H] DAMGO to bind to membranes of hippocampus, corpus striatum, pons and medulla, cortex and spinal cord of WKY and SHR rats did not differ. The Bmaxvalue of [3H] DAMGO in membranes of hypothalamus and midbrain of SHR rats was significantly higher than in WKY rats but the Kdvalues in the two strains did not differ. On the other hand, the Bmaxvalue of [3H] DAMGO in membranes of amygdala of SHR rats was lower than that of WKY rats but the Kdvalues in the two strains were similar. It is concluded that SHR rats have higher density of μ-opiate receptors in hypothalamus and midbrain but lower density in amygdala in comparison with WKY rats, and that such differences in the distribution of μ-opiate receptors may be related to the elevated blood pressure in SHR rats.