HEPATITIS-C VIRUS PARTICLE DETECTED BY IMMUNOELECTRON MICROSCOPIC STUDY
HEPATITIS-C VIRUS PARTICLE DETECTED BY IMMUNOELECTRON MICROSCOPIC STUDY
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DOI：
10.1099/0022-1317-75-7-1755
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发表时间：
1994-07-01
影响因子：
3.8
通讯作者：
KOHARA, M
中科院分区：
文献类型：
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作者：
KAITO, M;WATANABE, S;KOHARA, M
为了阐明丙型肝炎病毒（HCV）的形态，间接免疫金电子显微镜研究进行了两个血浆样品与高HCV RNA滴度使用多克隆和单克隆抗体特异性的推定的HCV包膜蛋白。蔗糖密度梯度离心后，在1.14至1.16 g/ml的组分中发现直径为55至65 nm的具有穗状突起的球形病毒样颗粒。这些颗粒仅在HCV感染的献血者中发现，其形态特征与黄病毒相似。此外，这些颗粒特异性反应的多克隆和单克隆抗体的推定的HCV包膜蛋白。这是第一个已知的报告，其中HCV颗粒的形态是清楚地显示。
To clarify the morphology of hepatitis C virus (HCV), an indirect immunogold electron microscopic study was carried out on two plasma samples with high HCV RNA titres using polyclonal and monoclonal antibodies specific to the putative HCV envelope protein. Spherical virus-like particles, 55 to 65 nm in diameter with spikelike projections, were found in 1.14 to 1.16 g/ml fractions after sucrose density gradient centrifugation. These particles were found only in HCV-infected blood donors and had morphological features similar to those of flaviviruses. Moreover, these particles specifically reacted with the polyclonal and monoclonal antibodies to the putative HCV envelope protein. This is the first known report in which the morphology of the HCV particle is clearly shown.