Feline mitochondrial DNA sampling for forensic analysis: when enough is enough!
Feline mitochondrial DNA sampling for forensic analysis: when enough is enough!
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DOI：
10.1016/j.fsigen.2014.11.017
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发表时间：
2015-05
影响因子：
3.1
通讯作者：
Lyons, Leslie A
中科院分区：
文献类型：
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作者：
Grahn, Robert A;Alhaddad, Hasan;Alves, Paulo C;Randi, Ettore;Waly, Nashwa E;Lyons, Leslie A
宠物毛发在解决法律问题方面具有明显的价值。猫的毛是长期脱落的，如果没有一定程度的二次转移，很难带着猫离开家。猫毛作为证据资源的力量可能没有得到充分利用，因为具有代表性的基因数据库不能用于排除目的。线粒体控制区数据库对于毛发分析是非常有价值的，并且已经为猫开发了。在一个具有代表性的全球数据集中，83%的国内猫有丝分裂型属于12个主要类型中的一个。在剩余的17%中，7.5%在已公布的1394样本数据库中是唯一的。目前的研究评估了建立家猫线粒体控制区法医比较代表性群体所需的样本量。对于大多数世界人口，随机抽样50个不相关的本地个体将达到95%的饱和度。99%的饱和度是通过随机抽样60-170只猫实现的，这取决于总体种群中可获得的有丝分裂型的数量。可能是由于最近猫的驯化和最小的本地化种群亚结构，比起人类或狗，满足线粒体DNA控制区数据库实际饱和所需的猫更少。再加上全球现有的近1400只猫的猫科动物控制区数据库，将需要最少的本地抽样来建立适当的比较代表性数据库，并实现显著的排斥力。
Pet hair has a demonstrated value in resolving legal issues. Cat hair is chronically shed and it is difficult to leave a home with cats without some level of secondary transfer. The power of cat hair as an evidentiary resource may be underused because representative genetic databases are not available for exclusionary purposes. Mitochondrial control region databases are highly valuable for hair analyses and have been developed for the cat. In a representative worldwide data set, 83% of domestic cat mitotypes belong to one of twelve major types. Of the remaining 17%, 7.5% are unique within the published 1394 sample database. The current research evaluates the sample size necessary to establish a representative population for forensic comparison of the mitochondrial control region for the domestic cat. For most worldwide populations, randomly sampling 50 unrelated local individuals will achieve saturation at 95%. The 99% saturation is achieved by randomly sampling 60–170 cats, depending on the numbers of mitotypes available in the population at large. Likely due to the recent domestication of the cat and minimal localized population substructure, fewer cats are needed to meet mitochondria DNA control region database practical saturation than for humans or dogs. Coupled with the available worldwide feline control region database of nearly 1400 cats, minimal local sampling will be required to establish an appropriate comparative representative database and achieve significant exclusionary power.