External Volume Expansion Increases Subcutaneous Thickness, Cell Proliferation, and Vascular Remodeling in a Murine Model
External Volume Expansion Increases Subcutaneous Thickness, Cell Proliferation, and Vascular Remodeling in a Murine Model
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DOI：
10.1097/prs.0b013e31825dc04d
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发表时间：
2012-09-01
影响因子：
3.6
通讯作者：
Orgill, Dennis P.
中科院分区：
文献类型：
--
作者：
Heit, Yvonne I.;Lancerotto, Luca;Orgill, Dennis P.
背景：脂肪移植是软组织重建的有力工具；然而，受体床准备背后的科学尚未得到彻底的探索。脂肪移植前抽吸外扩容技术已应用于临床，以提高移植物存活的可靠性和一致性。作者建立了一个小鼠模型来研究外体积扩张的潜在机制。方法：作者利用软硅胶圆顶连接到真空源(25 Mm Hg)来制作外体积扩张装置来治疗小鼠的背部，并将其反应与闭塞敷料治疗进行比较。治疗区域用磁共振成像进行监测。术后第7天用腐蚀铸造法观察微血管重塑情况，第28天观察对组织厚度、脂肪细胞数、细胞增殖及血管密度的影响。结果：肉眼观察显示，体外扩容装置处理后21d处组织肿胀，未见皮肤损伤。在第28天，外部体积扩张使皮下脂肪层厚度增加了两倍，这与磁共振成像观察一致。扩张处理区皮下组织增殖率增加2倍，柱状脂肪细胞数净增加2.2倍；血管网重塑，血管管型改变，管径增大，血管密度增大1.9倍。结论：体外扩容可诱导小鼠皮下组织高度增殖和血管化。使用外部体积扩张装置的受体部位准备可能是一种很有前途的增强细胞和组织植入的工具。(最后一次。重新构造外科手术130：541,2012。)
Background: Fat grafting is a powerful tool for soft-tissue reconstruction; however, the science behind recipient bed preparation has not been thoroughly explored. External volume expansion using suction before fat grafting has been used clinically to improve reliability and consistency of graft survival. The authors developed a murine model to investigate the underlying mechanism of external volume expansion.Methods: The authors created an external volume expansion device using a soft-silicone dome connected to a vacuum source (25 mmHg) to treat the dorsum of mice, and the response was compared with treatment with an occlusive dressing. Treated areas were monitored with magnetic resonance imaging. Remodeling of microvasculature was studied with corrosion casting on day 7. Effects on tissue thickness, number of adipocytes, cell proliferation, and blood vessel density were analyzed at 28 days.Results: Macroscopic analysis showed tissue swelling at sites treated with the external volume expansion device by 21 days, without skin damage. On day 28, external volume expansion increased the thickness of the subcutaneous fat layer twofold, consistent with magnetic resonance imaging observations. The proliferation rate in the subcutaneous layer of expansion-treated areas increased twofold, with a net 2.2-fold increase in number of adipocytes in columns; remodeling of the vessels network occurred, with reorientation and increase of vessel diameters shown by corrosion casting and 1.9-fold augmentation of vessels density.Conclusions: External volume expansion applied to mouse integument induces highly proliferative and vascularized subcutaneous tissue. Recipient-site preparation using external volume expansion devices may be a promising tool to enhance cell and tissue engraftment. (Plast. Reconstr. Surg. 130: 541, 2012.)