Influence of surface roughness on the initial formation of biofilm
Influence of surface roughness on the initial formation of biofilm
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DOI：
10.1016/j.surfcoat.2015.07.062
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发表时间：
2015-12-25
影响因子：
5.4
通讯作者：
Chen, Jinju
中科院分区：
文献类型：
--
作者：
Ammar, Yasmine;Swailes, David;Chen, Jinju
表面粗糙度是影响细胞在生物膜形成之前附着到表面的一个重要因素。纳米级粗糙表面的存在降低了细菌细胞接近表面时遇到的物理化学势垒。这项工作研究了废水处理生物膜形成之前的初始细菌沉积。这项工作的目的是模拟细菌底物和附件的表面粗糙度对细菌黏附的影响。在本研究中，根据扩展的Derjaguin-Landau-Verwey-Overbeek(DLVO)理论，细菌被视为附着在表面的惰性粒子。表面粗糙度是通过使用原子力显微镜(AFM)粗糙度分析得出的统计参数重建表面形貌来建模的。通过比较不同离子强度下在平行流室中测得的沉降量与计算流体动力学(CFD)的结果，验证了该模型的有效性。基于CFD模拟，建立了沉积速率与纳米级表面粗糙度之间的关系。这些结果可用于指导污水处理厂控制沉积的床料表面设计。(C)2015爱思唯尔B.V.保留所有权利。
Surface roughness is an important factor affecting cellular attachment to surfaces prior to biofilm formation. The presence of nanoscale asperities reduces the physicochemical potential barrier encountered by a bacterial cell when it approaches the surface. This work studies the initial bacterial deposition prior to biofilm formation for wastewater treatment. The objective of this work is to model how surface roughness of the substrate and appendages of the bacteria affect bacterial adhesion.In this study, bacteria are treated as inert particles that attach to surfaces according to the extended Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. Surface roughness is modelled by reconstructing the surface topography using statistical parameters derived from Atomic Force Microscopy (AFM) roughness analyses. The model is validated by comparing deposition measured in a parallel flow chamber to the Computational Fluid Dynamics (CFD) results for different ionic strengths. Based on CFD simulations, the relationship between the deposition rate and the nanoscale surface roughness has been established. These findings can be used to guide the surface design of bed materials in wastewater treatment plants to control deposition. (C) 2015 Elsevier B.V. All rights reserved.