Formation of Biocompatible Nanocapsules with Emulsion Core and Pegylated Shell by Polyelectrolyte Multi layer Adsorption
Formation of Biocompatible Nanocapsules with Emulsion Core and Pegylated Shell by Polyelectrolyte Multi layer Adsorption
复制标题
DOI：
10.1021/la102061s
复制
发表时间：
2010-08-03
期刊：
影响因子：
3.9
通讯作者：
Warszynski, P.
中科院分区：
文献类型：
--
作者：
Szczepanowicz, K.;Hoel, H. J.;Warszynski, P.
本工作的目的是开发一种基于生物相容性聚电解质（PE）多层吸附液芯封装的负载纳米胶囊的新方法，最外层有或没有聚乙二醇化。采用AOT （docusate sodium salt）作为乳化剂，制备了由AOT/PLL（聚l -赖氨酸氢溴化物）表面络合物稳定的岩心。这些带正电荷的核被聚电解质、生物相容性聚阴离子PGA（聚l -谷氨酸钠盐）和生物相容性聚阳离子PLL逐层吸附包裹。我们采用饱和法制备了连续层，并确定了表面活性剂和聚电解质的最佳浓度，以形成稳定的壳层。所得胶囊的平均粒径为60 nm。采用PGA-g-PEG（聚乙二醇接枝的PGA）制备聚乙二醇化外膜。该胶囊至少在3个月内稳定。在细胞共培养实验中，这些纳米胶囊具有生物相容性，在流式细胞术测试中，这些纳米胶囊与外周血单个核细胞没有或非常低的非特异性结合。为了研究药物对白血病细胞的作用，β -胡萝卜素和维生素A被封装为模型药物。
The aim of this work was to develop a novel method of preparation of loaded nanosize capsules based on liquid core encapsulation by biocompatible polyelectrolyte (PE) multilayer adsorption, with or without pegylated outermost layer. Using AOT (docusate sodium salt) as emulsifier, we obtained cores, stabilized by an AOT/PLL (poly-L-lysine hydrobromide) surface complex. These positively charged cores were encapsulated by layer-by-layer adsorption of polyelectrolytes, biocompatible polyanion PGA (poly-L-glutamic acid sodium salt), and biocompatible polycation PLL. We used the saturation method for formation of consecutive layers, and we determined the optimal conditions concerning concentration of surfactant and polyelectrolytes to form stable shells. The average size of the obtained capsules was 60 nm. Pegylated external layer were prepared using PGA-g-PEG (PGA grafted by PEG poly(ethylene glycol)). The capsules were stable for at least a period of 3 months. These nanocapsules were biocompatible when tested for cytotoxicity in a cellular coculture assay and demonstrated no or very low nonspecific binding to peripheral blood mononuclear cells when tested by flow cytometry. In order to study drug effects on leukemia cells, beta-carotene and vitamin A have been encapsulated as model drugs.