CHARACTERIZATION OF BIOLOGIC PROPERTIES OF WOUND FLUID COLLECTED DURING EARLY STAGES OF WOUND-HEALING
CHARACTERIZATION OF BIOLOGIC PROPERTIES OF WOUND FLUID COLLECTED DURING EARLY STAGES OF WOUND-HEALING
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DOI：
10.1111/1523-1747.ep12667378
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发表时间：
1992-11-01
影响因子：
6.5
通讯作者：
LYDON, MJ
中科院分区：
文献类型：
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作者：
CHEN, WYJ;ROGERS, AA;LYDON, MJ
封闭性敷料对伤口愈合的临床影响已得到充分记录。然而，与湿润愈合相关的潜在生物学机制尚未得到很好的理解。实验研究和临床经验已经显示，在闭塞下增强的焦痂和凝块去除、再上皮化和胶原蛋白合成，表明蛋白酶和其他效应物的活性升高的可能性，例如，生长因子，在潮湿的伤口环境中。为了深入了解闭塞下早期伤口的生物学，我们对闭塞的全层和部分层伤口的液体进行了生物学和生物化学分析。在伤口液样品中检测金属蛋白酶活性。当应用于培养的真皮成纤维细胞时，观察到全层伤口液体的促有丝分裂活性。伤口液刺激的尿激酶型纤溶酶原激活剂的成纤维细胞的积累也观察到在一个时间依赖性的方式。还观察到成纤维细胞对金属蛋白酶积累的刺激。我们通过抗体中和它们的生物活性，进一步证明了血小板衍生生长因子样因子和碱性成纤维细胞生长因子样因子在伤口液中的存在。与非封闭的“干燥”伤口相比，封闭伤口中保留的伤口液的蛋白酶存在和蛋白酶刺激活性可能有助于这些伤口中蛋白水解环境的增强。生长因子的存在和蛋白酶激活潜在生长因子并通过结缔组织分解产生趋化肽的潜在能力也可能有助于促进闭塞伤口的愈合。
The clinical effects of occlusive dressings on wound healing are well documented. However, the underlying biologic mechanisms associated with moist healing are not well understood. Experimental studies and clinical experience have shown enhanced eschar and clot removal, re-epithelialization, and collagen synthesis under occlusion, suggesting the possibility of elevated activities of proteinases and other effectors, e.g., growth factors, in the moist wound environment. To gain an insight into the biology of early wounds under occlusion, we have carried out biologic and biochemical analyses on fluids from occluded full- and partial-thickness wounds. Metalloproteinase activities were detected in the wound fluid samples. When applied to cultured dermal fibroblasts, mitogenic activity was observed with fluids from full-thickness wounds. Wound fluid - stimulated accumulation of urokinase-type plasminogen activator by fibroblasts was also observed in a time-dependent manner. Stimulation of metalloproteinase accumulation by fibroblasts was also observed. We have further demonstrated the presence of platelet-derived growth factor-like and basic fibroblast growth factor - like factors in wound fluid by antibody neutralization of their biologic activities. Proteinase presence and proteinase stimulatory activity of wound fluid retained in the occluded wound may contribute to an enhanced proteolytic environment in these wounds in comparison to non-occluded "dry" wounds. The presence of growth factors and the potential abilities of proteinases to activate latent growth factors and generate chemotactic peptides through connective tissue breakdown may also contribute to the enhanced healing of occluded wounds.