H~+ Involving Signal Transduction in Regulation of Vicia faba L. Stomatal Movement by Jasmonic Acid
H~+ Involving Signal Transduction in Regulation of Vicia faba L. Stomatal Movement by Jasmonic Acid
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发表时间：
2007
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通讯作者：
Meng Fan
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作者：
Meng Fan
利用荧光探针BCECF-AM和Fluo-3在共聚焦显微镜下监测蚕豆保护细胞中pHi和[Ca2+]cyt的变化。研究了H+是否参与茉莉酸调节气孔运动的信号转导。结果表明，在0.1~100 μmol·L-1浓度下，（-）JA诱导气孔关闭，并介导保护细胞胞内碱化。相比之下，（±）JA诱导的气孔开度没有pHi变化。药理实验证明，Na3VO4降低了（-）JA诱导的气孔关闭的有效性，而梭菌素对（±）JA诱导的气孔打开没有影响。（-）JA和（±）JA诱导[Ca2+]cyt的变化比较：（-）JA诱导[Ca2+]cyt升高较快，（±）JA诱导[Ca2+]cyt升高和降低缓慢。这些结果表明（-）JA和（±）JA在调节气孔运动中具有不同的作用和信号通路。
Using fluorescent probe BCECF-AM and Fluo-3 monitored the changes of pHi and [Ca2+]cyt in guard cells of Vicia faba under confocal microscopy. Whether H+ was involved in signal transduction in JA regulating stomatal movement was tested. The results showed that (–)JA induced stomatal closure at concentrations of 0.1~100 μmol·L-1 was mediated intracellular alkalization in guard cells. In contrast, (±)JA induced stomatal open without pHi changes. Pharmacological experiment proved that Na3VO4 reduced the effectiveness of (–)JA- induced stomatal closure, while fusicoccin had no effect on (±)JA-induced stomatal opening. Compared changes of [Ca2+]cyt induced by (–)JA and (±)JA: (–)JA increased [Ca2+]cyt rapidly, while (±)JA induced [Ca2+]cyt increas- ing and decreasing slowly. These results demonstrated that (–)JA and (±)JA had different roles and signal pathways in regulation of stomatal movement.