Burkholderia bacteria use chemotaxis to find social amoeba Dictyostelium discoideum hosts
Burkholderia bacteria use chemotaxis to find social amoeba Dictyostelium discoideum hosts
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DOI：
10.1038/s41396-018-0147-4
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发表时间：
2018-08-01
期刊：
影响因子：
11
通讯作者：
Strassmann, Joan E.
中科院分区：
文献类型：
--
作者：
Shu, Longfei;Zhang, Bojie;Strassmann, Joan E.
合作中的一个关键问题是如何找到合适的合作伙伴并保持合作关系。这对于水平转移的细菌共生体来说尤其具有挑战性，因为它们必须反复重新建立关系。在社会性变形虫Dictyosteelium discoideum养殖共生体中，两种不可食用的伯克霍尔德氏菌（Burkholderia agricolaris和Burkholderia hayleyella）与原生D.盘状体宿主并导致携带额外的细菌物种。然而，目前尚不清楚D.盘状芽孢杆菌及其携带的伯克霍尔德氏菌得以形成和维持。在这里，我们来看看伯克霍尔德氏菌是如何找到宿主的。结果表明，从D. discoideum，而不是其他测试的伯克霍尔德氏菌物种被吸引到D。盘状体上清液，表明这种结合不仅仅是阿米巴随意吞噬的结果。趋化反应受到双方的影响。我们发现证据表明B. hayleyella偏爱D. discoideum克隆，目前或以前携带伯克霍尔德氏菌，而B。agricolaris没有表现出这种偏好。然而，我们没有发现伯克霍尔德氏菌对自己的宿主克隆或自己物种的其他宿主的偏好的证据。我们进一步研究了D.可以使用基于质谱的代谢组学方法来解释上面显示的模式。这些结果表明，这些细菌共生体能够优先找到并在一定程度上选择它们的单细胞伴侣。此外，这项研究还表明，细菌可以主动搜索和靶向吞噬细胞，这可能有助于我们更好地了解细菌如何与免疫系统相互作用。
A key question in cooperation is how to find the right partners and maintain cooperative relationships. This is especially challenging for horizontally transferred bacterial symbionts where relationships must be repeatedly established anew. In the social amoeba Dictyostelium discoideum farming symbiosis, two species of inedible Burkholderia bacteria (Burkholderia agricolaris and Burkholderia hayleyella) initiate stable associations with naive D. discoideum hosts and cause carriage of additional bacterial species. However, it is not clear how the association between D. discoideum and its carried Burkholderia is formed and maintained. Here, we look at precisely how Burkholderia finds its hosts. We found that both species of Burkholderia clones isolated from D. discoideum, but not other tested Burkholderia species, are attracted to D. discoideum supernatant, showing that the association is not simply the result of haphazard engulfment by the amoebas. The chemotactic responses are affected by both partners. We find evidence that B. hayleyella prefers D. discoideum clones that currently or previously carried Burkholderia, while B. agricolaris does not show this preference. However, we find no evidence of Burkholderia preference for their own host clone or for other hosts of their own species. We further investigate the chemical differences of D. discoideum supernatants that might explain the patterns shown above using a mass spectrometry based metabolomics approach. These results show that these bacterial symbionts are able to preferentially find and to some extent choose their unicellular partners. In addition, this study also suggests that bacteria can actively search for and target phagocytic cells, which may help us better understand how bacteria interact with immune systems.