Online photocatalytic device for highly selective pre-column fluorescence derivatization of 5-hydroxyindoles with benzylamine
Online photocatalytic device for highly selective pre-column fluorescence derivatization of 5-hydroxyindoles with benzylamine
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5-羟基吲哚与苄胺高选择性柱前荧光衍生在线光催化装置
DOI：
10.1016/j.aca.2005.08.076
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发表时间：
2006
影响因子：
6.2
通讯作者：
M. Yamaguchi
中科院分区：
文献类型：
--
作者：
K. Todoroki;Y. Nakashima;H. Yoshida;H. Nohta;M. Yamaguchi
建立了一种基于在线光催化氧化介导的苯胺衍生化过程的测定5-羟基吲哚的荧光液相色谱方法。在这项研究中，我们使用了一个由tefzel管组成的光催化柱，其中填充了二氧化钛涂层玻璃珠，作为柱前衍生化反应器。在近紫外照射下，5-羟基吲哚通过反应柱时，用苯胺进行了荧光衍生化反应。采用含有3mM辛烷磺酸钠的100mM醋酸缓冲液(pH 4.6) -乙腈（72:28,v/v，等密度洗脱），在50min内反相液相色谱连续分离5-羟基吲哚衍生物；在350nm处激发，在465nm处荧光检测样品。5-羟基吲哚的检出限（信噪比为3）在160 ~ 360fmol / 5μL。我们已将该方法应用于人体尿液中5-羟基吲哚-3-乙酸的测定，该方法需要最少的样品预处理。
A fluorimetric liquid chromatographic method for the determination of 5-hydroxyindoles based on the benzylamine derivatization process mediated through an online photocatalytic oxidation has been developed. In this study, we used a photocatalytic column comprising tefzel tubing packed with TiO2-coated glass beads, as a pre-column derivatization reactor. The fluorescence derivatization of 5-hydroxyindoles using benzylamine proceeded during their passage through the reaction column under near-UV irradiation. The 5-hydroxyindole derivatives were separated continuously on a reversed-phase liquid chromatography within 50min, using 100mM acetate buffer (pH 4.6)–acetonitrile (72:28, v/v; isocratic elution) containing 3mM sodium octanesulfonate; the samples were detected fluorimetrically at 465nm upon excitation at 350nm. The detection limits (signal-to-noise ratio=3) of the 5-hydroxyindoles were in the range from 160 to 360fmol per 5μL injection. We have applied this method, which requires minimal sample pre-treatment, to the determination of 5-hydroxyindole-3-acetic acid in human urine.