Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics
Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics
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DOI：
10.1126/science.aac7745
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发表时间：
2015-10-23
期刊：
影响因子：
56.9
通讯作者：
Tyson, Gene W.
中科院分区：
文献类型：
--
作者：
Evans, Paul N.;Parks, Donovan H.;Tyson, Gene W.
产甲烷和产甲烷营养古菌在全球甲烷通量中起着重要作用。不依赖培养的方法为甲烷代谢微生物的多样性和进化提供了更深入的了解，但是，直到现在，还没有令人信服的证据表明在Euryarchaeota门之外的古细菌中存在甲烷代谢。我们对一个深层含水层进行了宏基因组测序，恢复了两个近乎完整的基因组，属于古细菌门深海archaeota（以前称为杂绿archaeotal Group）。这些基因组包含甲烷代谢所必需的不同同源基因，包括那些编码甲基辅酶M还原酶（MCR）复合物的基因。在一系列环境中发现的其他非euryarchaeo mcr编码基因表明，未被识别的古细菌谱系也可能有助于全球甲烷循环。这些发现表明甲烷代谢在Euryarchaeota和Bathyarchaeota的最后一个共同祖先之前就已经出现了。
Methanogenic and methanotrophic archaea play important roles in the global flux of methane. Culture-independent approaches are providing deeper insight into the diversity and evolution of methane-metabolizing microorganisms, but, until now, no compelling evidence has existed for methane metabolism in archaea outside the phylum Euryarchaeota. We performed metagenomic sequencing of a deep aquifer, recovering two near-complete genomes belonging to the archaeal phylum Bathyarchaeota (formerly known as the Miscellaneous Crenarchaeotal Group). These genomes contain divergent homologs of the genes necessary for methane metabolism, including those that encode the methyl-coenzyme M reductase (MCR) complex. Additional non-euryarchaeotal MCR-encoding genes identified in a range of environments suggest that unrecognized archaeal lineages may also contribute to global methane cycling. These findings indicate that methane metabolism arose before the last common ancestor of the Euryarchaeota and Bathyarchaeota.