Changes in predominant bacterial populations in human faeces with age and with Clostridium difficile infection
Changes in predominant bacterial populations in human faeces with age and with Clostridium difficile infection
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DOI：
10.1099/0022-1317-51-5-448
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发表时间：
2002-05-01
影响因子：
3
通讯作者：
MacFarlane, GT
中科院分区：
文献类型：
--
作者：
Hopkins, MJ;MacFarlane, GT
人类粪便中的细菌组成可能因年龄和疾病等因素而变化很大，尽管相对较少的研究监测了这些事件，特别是在物种水平上。在本研究中，从健康年轻人和老年受试者以及患有艰难梭菌相关腹泻（CDAD）的老年患者的粪便样本中分离细菌。根据细胞脂肪酸谱，采用生物体识别系统对微生物进行种属水平鉴定。在某些细菌类群中，尽管生物体的总体数量在属水平上相似，但物种多样性仍随年龄而变化。Taitaomicron，B.卵形菌和单宁普雷沃菌是从青壮年分离的常见革兰氏阴性厌氧菌。健康老年人粪便中拟杆菌物种多样性增加。双歧杆菌物种多样性随着年龄的增长而下降，双歧杆菌和Bif。angulatum是最常见的分离物。CDAD患者的特点是兼性菌群、乳酸杆菌和梭菌的多样性更大，但类杆菌、普雷沃菌和双歧杆菌的数量大大减少。正常微生物群中的这种细菌种群变化可能导致有利于致病微生物（例如梭菌）建立的代谢条件，并且将对整个大肠的生化能力产生相当大的影响。双歧杆菌和乳酸杆菌群落结构的改变与饮食和治疗干预有关，例如使用益生菌或益生菌，旨在以有益的方式改变肠道微生物菌群的组成或代谢活性，特别是在老年人或有CDAD风险的个体中。
The bacterial composition of human faeces can vary greatly with factors such as age and disease, although relatively few studies have monitored these events, particularly at species level. In this investigation, bacteria were isolated from faecal samples from healthy young adults and elderly subjects, and elderly patients with Clostridium difficile-associated diarrhoea (CDAD). The organisms were identified to species level on the basis of their cellular fatty acid profiles with the MIDI system. In some groups of bacteria, species diversity was found to change with age despite the overall numbers of organisms being similar at genus level. Bacteroides thetaitaomicron, B. ovatus and Prevotella tannerae were common gram-negative anaerobes isolated from young adults. Bacteroides species diversity increased in the faeces of healthy elderly people. Bifidobacterial species diversity decreased with age, with Bifidobacterium adolescentis and Bif. angulatum being the most common isolates. CDAD patients were characterised by greater diversity of facultative species, lactobacilli and clostridia, but greatly reduced numbers of bacteroides, prevotella and bifidobacteria. Such bacterial population changes in the normal microbiota could result in metabolic conditions favourable for the establishment of pathogenic micro-organisms, such as clostridia, and would have considerable effects on the biochemical capacity of the large intestine as a whole. Alterations in the community structure of bifidobacteria and lactobacilli have relevance for dietary and therapeutic interventions such as the use of pre- or probiotics that aim to modify the composition or metabolic activities of the intestinal microflora in a beneficial way, particularly in elderly people or individuals at risk of CDAD.