Net Shape Nonwoven: a novel technique for porous three-dimensional nonwoven hybrid scaffolds
Net Shape Nonwoven: a novel technique for porous three-dimensional nonwoven hybrid scaffolds
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DOI：
10.1177/0040517513515315
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发表时间：
2014-06-01
影响因子：
2.3
通讯作者：
Hanke, Thomas
中科院分区：
文献类型：
--
作者：
Hild, Martin;Bruenler, Ronny;Hanke, Thomas
由生物相容性、骨传导性和可吸收的壳聚糖丝制成的纺织结构为骨组织工程应用的支架提供了极好的先决条件。描述了新颖的网状非织造（NSN）技术，该技术能够将短纤维加工成具有可调节孔径分布的三维网状非织造结构。制备了由纯壳聚糖纤维制成的 NSN 支架。通过将 NSN 技术分别与静电纺丝和胶原蛋白浸涂相结合，实现了改善初始细胞粘附的 NSN 混合支架。扫描电子显微镜和液体置换孔隙度测定法揭示了互连的开孔支架结构。新型壳聚糖混合支架为接种的人骨髓基质细胞的粘附、增殖和分化提供了适当的条件，证明它们适合用于硬组织再生。
Textile structures made of biocompatible, osteoconductive and resorbable chitosan-filaments provide excellent preconditions as scaffolds for Bone Tissue Engineering applications. The novel Net Shape Nonwoven (NSN) technique that enables short fibers to be processed into three-dimensional net-shaped nonwoven structures with adjustable pore size distributions is described. NSN scaffolds made of pure chitosan fibers were fabricated. NSN hybrid scaffolds for improved initial cell adhesion were realized by combining the NSN technique with electrospinning and dip-coating with collagen, respectively. Scanning electron microscopy and liquid displacement porosimetry revealed an interconnecting open porous scaffold structure. The novel chitosan-hybrid scaffolds provide proper conditions for adhesion, proliferation and differentiation of the seeded human bone marrow stromal cells, proving that they are suitable for usage in hard-tissue regeneration.