Ionic charge on phospholipids and their interaction with the mitochondrial adenosine triphosphatase.
Ionic charge on phospholipids and their interaction with the mitochondrial adenosine triphosphatase.
复制标题
磷脂上的离子电荷及其与线粒体三磷酸腺苷酶的相互作用。
DOI：
10.1016/0005-2736(79)90124-x
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
Govit Sinthusek
中科院分区：
文献类型：
--
作者：
Carol C. Cunningham;Govit Sinthusek
在磷脂酰甘油和磷脂酰甘油赖氨酸混合物制备的脂质体存在下，测定了脂质耗竭、寡霉素敏感的线粒体ATPase的活性。由磷脂酰甘油-磷脂酰甘油赖氨酸混合物制备的脂质体的酶活性随着负电荷的增加而线性增加。测定了其他几种磷脂的电泳率和活化能力。脂质体的凝胶迁移率和寡霉素敏感活性之间的线性关系再次明显。这些观察表明，如果磷酸甘油酯的酰基链组成相对恒定，则ATPase的活性与磷脂激活剂上的离子电荷成正比。
The activity of the lipid-depleted, oligomycin-sensitive mitochondrial ATPase has been measured in the presence of liposomes prepared from mixtures of phosphatidylglycerol and phosphatidylglycerol lysine. Enzyme activity increased linearly with an increase in the negative charge of liposomes prepared from the phosphatidylglycerol-phosphatidylglycerol lysine mixtures. The electrophoretic mobility and activating capacity of liposomes of several other phospholipids were determined. A linear relationship between electrophoretic mobility of the liposomes and oligomycin-sensitive activity was again apparent. These observations demonstrate that the activity of the ATPase is directly proportional to the ionic charge on phospholipid activators if the acyl chain composition of the phosphoglycerides is relatively constant.