Interplay between polymorphisms and methylation in the H19/IGF2 gene region may contribute to obesity in Mexican-American children.
Interplay between polymorphisms and methylation in the H19/IGF2 gene region may contribute to obesity in Mexican-American children.
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DOI：
10.1017/s204017441300041x
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发表时间：
2013-12
影响因子：
1.7
通讯作者：
Gorlova O
中科院分区：
文献类型：
--
作者：
Hernández-Valero MA;Rother J;Gorlov I;Frazier M;Gorlova O
印记基因常常影响与体型相关的特征，比如体重。然而，印记与肥胖，尤其是儿童肥胖之间的关联尚未得到充分研究。墨西哥裔美国儿童肥胖和/或超重的患病率很高，接近50%。在一项针对75名墨西哥裔美国儿童的初步研究中，我们分析了印记的H19/IGF2基因座的差异甲基化区域（DMR）中一个CpG位点的肥胖/超重状况、甲基化状态以及单核苷酸多态性（SNP）状态之间的关系。我们观察到在超重和肥胖儿童中，男孩和女孩之间SNP rs10732516的频率存在显著差异，但在偏瘦儿童中没有这种差异。我们还发现，H19 DMR中多态性CpG位点（CpG4）甲基化程度较低的儿童比甲基化程度较高的儿童出生体重更高（P = 0.04）。我们的研究结果表明，CpG4甲基化状态可能以性别特异性的方式与墨西哥裔美国儿童的儿童肥胖相关。
Imprinted genes often affect body size-related traits such as weight. However, the association of imprinting with obesity, especially childhood obesity, has not been well studied. Mexican-American children have a high prevalence, approaching 50%, of obesity and/or overweight. In a pilot study of 75 Mexican-American children, we analyzed the relationships among obese/overweight status, methylation status and single-nucleotide polymorphism (SNP) status at a CpG site in a differentially methylated region (DMR) of the imprinted H19/IGF2 locus. We observed a significant difference in SNP rs10732516 frequency between boys and girls among the overweight and obese children but not among the lean children. We also found that children with lower methylation of the polymorphic CpG site (CpG4) in the H19 DMR had higher birth weights than did children with higher methylation (P = 0.04). Our results suggest that CpG4 methylation status may be associated with childhood obesity in Mexican-American children in a sex-specific manner.