NEONATAL MUSCLE - ELECTRON-MICROSCOPIC STUDY
NEONATAL MUSCLE - ELECTRON-MICROSCOPIC STUDY
复制标题
DOI：
10.1002/ar.1091890410
复制
发表时间：
1977-01-01
影响因子：
--
通讯作者：
ONTELL, M
中科院分区：
文献类型：
--
作者：
ONTELL, M
对2日龄大鼠趾长伸肌进行间隔连续切片，EM研究，以确定是否所有楔入肌纤维的基膜和肌膜之间的细胞，无论其形态如何，都是真正的肌卫星细胞。肌肉相对不成熟，包含封闭在共同基底膜（簇）中的细胞群，并显示原始肌神经连接。肌纤维团中的肌细胞包括初生纤维、卫星纤维、不同发育阶段的肌管和肌卫星细胞。在单切片中，一些早期肌管缺乏肌丝，难以与肌卫星细胞区分。间隔连续切片显示，所有肌卫星细胞有异染色质核和高的核/质比，所有苍白染色的细胞核中发现一个较低的核/质比，含有可变数量的肌丝。除了肌细胞外，肥大细胞、成纤维细胞样细胞和其他未分类的细胞全部或部分被包裹在簇的基底膜内。单独的位置或位置和细胞的形态学外观在一个单一的切片未能提供足够的标准，以正确分类所有的细胞在新生儿肌肉群。
A spaced serial section, EM study of the extensor digitorum longus of the 2 day old rat was undertaken to determine whether all cells wedged between the basement membrane and the sarcolemma of a muscle fiber, regardless of their morphology, were true myosatellite cells. The muscle was relatively immature, containing groups of cells enclosed in a common basement membrane (clusters) and exhibiting primitive myoneural junctions. Cells of the muscle line included in the clusters were primary fibers, satellite fibers, myotubes in different stages of development and myosatellite cells. In single ultrathin sections, some early myotubes lacked myofilaments and were difficult to distinguish from myosatellite cells. Spaced serial sections revealed that all myosatellite cells had heterochromatic nuclei and a high nuclear/cytoplasmic ratio and that all pale staining nuclei were found in cells with a lower nuclear/cytoplasmic ratio, containing variable quantities of myofilaments. In addition to the cells of the muscle line, mast cells, fibroblast-like cells and other unclassified cells were entirely or partially enclosed within the cluster''s basement membrane. Location alone or location and the morphological appearance of cells in a single ultrathin section failed to provide sufficient criteria to properly classify all of the cells found in neonatal muscle clusters.