DIFFERENTIATION OF LEUKEMIA-CELLS TO POLYMORPHONUCLEAR LEUKOCYTES IN PATIENTS WITH ACUTE NONLYMPHOCYTIC LEUKEMIA
DIFFERENTIATION OF LEUKEMIA-CELLS TO POLYMORPHONUCLEAR LEUKOCYTES IN PATIENTS WITH ACUTE NONLYMPHOCYTIC LEUKEMIA
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DOI：
10.1056/nejm198607033150103
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发表时间：
1986-07-03
影响因子：
158.5
通讯作者：
VOGELSTEIN, B
中科院分区：
文献类型：
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作者：
FEARON, ER;BURKE, PJ;VOGELSTEIN, B
我们使用重组DNA技术来确定急性非淋巴细胞白血病患者在发病、缓解和复发时多形核白细胞的来源。使用X染色体连锁DNA多态性的研究强烈表明，这种形式的白血病中的白血病母细胞可以在体内分化形成成熟的粒细胞。在母细胞中常见的染色体变化，如8号染色体的增加或7号染色体的丢失，被发现存在于白血病患者的成熟粒细胞中。此外，免疫球蛋白重链基因重排检测到在一个病人的多形核白细胞。最后，我们观察到13例完全缓解患者中有3例的粒细胞中持续存在一个单一的显性造血克隆。这些研究结果表明，重组DNA探针可以检测急性非淋巴细胞白血病患者的粒细胞克隆群体，并提供进一步的证据，在一些患者白血病母细胞分化形成成熟细胞。
We used recombinant-DNA techniques to determine the origin of polymorphonuclear leukocytes in patients with acute nonlymphocytic leukemia, at presentation, in remission, and in relapse. Studies using X chromosome-linked DNA polymorphisms strongly suggested that leukemic blast cells in this form of leukemia can differentiate in vivo to form mature granulocyte. Common chromosomal changes seen in blast cells, such as the addition of a chromosome 8 or the loss of a chromosome 7, were found to be present in the mature granulocytes of patients with leukemia. In addition, rearrangement of the immunoglobulin heavy-chain gene was detected in the polymorphonuclear leukocytes of one patient. Finally, we have observed the persistence of a single dominant hemopoietic clone in the granulocytes of 3 of 13 patients in complete remission. These findings demonstrate that recombinant-DNA probes can detect clonal populations of granuloctyes in patients with acute nonlymphocytic leukemia, and provide further evidence that in some patients leukemic blasts cells differentiate to form mature cells.