Attenuation of p47(phox) and p67(phox) membrane translocation as the inhibitory mechanism of S-nitrosothiol on the respiratory burst oxidase in human neutrophils
Attenuation of p47(phox) and p67(phox) membrane translocation as the inhibitory mechanism of S-nitrosothiol on the respiratory burst oxidase in human neutrophils
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DOI：
10.1006/bbrc.1996.0351
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发表时间：
1996-03-07
影响因子：
3.1
通讯作者：
Park, JW
中科院分区：
文献类型：
--
作者：
Park, JW
本文用体外易位系统研究了S-亚硝基硫醇（RSNO）对人中性粒细胞NADPH氧化酶的激活作用，在该系统中，阴离子两亲性物质，如十二烷基硫酸钠或花生四烯酸，起着激活剂的作用。当用RSNO预处理的膜和来自静息中性粒细胞的胞质组分组合以重建NADPH氧化酶时，胞质NADPH氧化酶组分如p47（phox）和p67（phox）向质膜组分的转运以及随后的超氧化物的产生都被抑制。而在酶活化后加入RSNO对O-2（-）的产生没有影响。用RSNO处理的膜观察到重组p47（phox）易位的类似抑制。当RSNO处理的膜部分暴露于2-巯基乙醇的抑制被逆转。这些数据表明RSNO通过直接作用于NADPH氧化酶的膜组分来抑制p47（phox）或含有p47（phox）的胞质复合物的易位。(C)出版社：Academic Press，Inc.
The effect of the S-nitrosothiol (RSNO) on the activation of NADPH oxidase in human neutrophils was studied using an in vitro translocation system in which an anionic amphiphil, such as sodium dodecyl sulfate or arachidonate, plays a role as an activator. When membranes pretreated with RSNO and a cytosol fraction from resting neutrophils were combined to reconstitute the NADPH oxidase, both translocation of the cytosolic NADPH oxidase components such as p47(phox) and p67(phox) to thr plasma membrane fraction and subsequent superoxide generation was inhibited. However, RSNO had no effect on O-2(-) production when added after enzyme activation. A similar inhibition of translocation of recombinant p47(phox) was observed with RSNO-treated membrane. When the RSNO-treated membrane fraction was exposed to 2-mercaptoethanol the inhibition was reversed. The data suggest that RSNO inhibits translocation of p47(phox) Or p47(phox) containing cytosolic complex via a direct effect on the membrane component of the NADPH oxidase. (C) 1996 Academic Press, Inc.