Dual-modal visual/photoelectrochemical all-in-one bioassay for rapid detection of AFP using 3D printed microreactor device
Dual-modal visual/photoelectrochemical all-in-one bioassay for rapid detection of AFP using 3D printed microreactor device
复制标题
使用 3D 打印微反应器装置快速检测 AFP 的双模式视觉/光电化学一体化生物测定
DOI：
10.1016/j.bios.2020.112158
复制
发表时间：
2020-06-15
影响因子：
12.6
通讯作者：
Gong, Jingming
中科院分区：
文献类型：
--
作者：
Li, Xin;Pan, Xinmeng;Gong, Jingming
构建了一种用于甲胎蛋白（AFP）比色和光电化学（PEC）检测的3D打印“一体化”双模式免疫分析（3D-AIO），其使用自动微流体将所有分步分析功能组件（包括免疫/酶反应、分离和检测）集成在一起。酶联免疫反应释放的抗坏血酸（AA）可将胱氨酸还原为半胱氨酸，诱导金纳米粒子（AuNPs）聚集，作为比色分析的报告试剂。同时，释放的AA诱导光活性纳米结构ZnIn 2S 4（ZIS）的空穴捕获，从而触发ZIS修饰的丝网印刷电极（标记为ZIS/SPE）在PEC检测室中开槽的显著光电流增强。通过智能控制，当血清中AFP含量超过其临界值（20 ng/mL）时，比色法显示出可区分的颜色变化，实现了对血浆样品进行快速筛查和快速鉴别的目的，特别是在床旁（POC）分析中。结果表明，PEC免疫分析法的检测限可达0.01ng/mL（-1）（S/N = 3）。该方法可实现AFP的快速定性筛查和PEC的定量检测，可满足POC快速、准确分析的要求。从人体血液中直接检测AFP使其有希望用于现场POC诊断。
An elaborated 3D printing "all-in-one" dual-modal immunoassay (3D-AIO) has been constructed for the colorimetric and photoelectrochemical (PEC) detection of alpha-fetoprotein (AFP), which integrates all step-analysis functional components (including immune/enzyme reaction, separation and detection) together using automatic microfluidics. The released ascorbic acid (AA) from the enzyme-linked immunoreactions can induce the aggregation of gold nanoparticles (AuNPs) by reducing cystine into cysteine, serving as the reporting agent of colorimetric assay. Meanwhile, the released AA induces hole-trapping of the photoactive nanostructured ZnIn2S4 (ZIS), thus triggering a noticeable photocurrent enhancement at ZIS modified screen printed electrode (labeled as ZIS/SPE) slotted in PEC detection chamber. By smart controlling, the colorimetric assays exhibits a distinguishable color change once AFP contents in serum exceed its cut-off value (20 ng mL(-1)), achieving fast screening and rapid identification purpose for plasma samples as negative or positive, especially in point of care (POC) analysis. And then the PEC immunoassay could be used for more accurate quantitative analysis with the detection limit as low as 0.01 ng mL(-1) (S/N = 3). The proposed assay offered bimodal readout for realizing both qualitative fast screening and quantitative PEC determination of AFP concentration, thereby meeting the requirements of quick and precise POC analysis. The direct detection of AFP from human blood makes it promising for on-site POC diagnostics.