ERGIC-53, A MEMBRANE-PROTEIN OF THE ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT, IS IDENTICAL TO MR60, AN INTRACELLULAR MANNOSE-SPECIFIC LECTIN OF MYELOMONOCYTIC CELLS
ERGIC-53, A MEMBRANE-PROTEIN OF THE ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT, IS IDENTICAL TO MR60, AN INTRACELLULAR MANNOSE-SPECIFIC LECTIN OF MYELOMONOCYTIC CELLS
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DOI：
10.1074/jbc.270.8.3551
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发表时间：
1995-02-24
影响因子：
4.8
通讯作者：
ROCHE, AC
中科院分区：
文献类型：
--
作者：
ARAR, C;CARPENTIER, V;ROCHE, AC
通过亲和层析从人骨髓单核细胞HL 60细胞分离的甘露糖特异性膜凝集素（MR 60）在未成熟单核细胞的细胞内细胞器中表达（Pimpaneau，V.，Midoux，P.，蒙席尼先生和Roche，A. C.（1991）碳水化合物。Res. 213，95-108）。它不存在于细胞表面，并且在免疫化学和结构上与成熟巨噬细胞的M（r）175，000甘露糖受体不同。MR 60 cDNA的分离和表征，其序列的基础上，MR 60是不相关的任何已知的哺乳动物凝集素。令人惊讶的是，发现MR 60与ERGIC-53相同（Schindler，R.，伊廷角，萨格勒布，M.，Lottspeich，F.，和Hauri，H. P.（1993）Eur. J. Cell Biol.61，1-9），一种I型整合膜蛋白，定义为在高尔基体和内质网之间再循环的中间隔室的标记物; MR 60/ERGIC-53与VIP-36具有重要的同源性，具有豆科植物凝集素（Fiedler，K.，和Simmons，R.（1994）Cell 77，625-626）。我们扩展了这些发现，证明MR 60/ERGIC-53和哺乳动物半乳糖凝集素（可溶性β半乳糖结合蛋白）之间的结构同源性。MR 60/ERGIC-53是第一个被鉴定为内质网-高尔基体蛋白的凝集素。因此，这种胞内甘露糖结合蛋白可能参与了内质网和高尔基体之间糖蛋白的运输。
A mannose specific membrane lectin (MR60) isolated from human myelomonocytic HL60 cells by affinity chromatography is expressed in intracellular organelles of immature monocytes (Pimpaneau, V., Midoux, P., Monsigny, M., and Roche, A. C. (1991) Carbohydr. Res. 213, 95-108). It is not present at the cell surface and is immunochemically and structurally distinct from the M(r) 175,000 mannose receptor of mature macrophages. MR60 cDNA was isolated and characterized; on the basis of its sequence, MR60 is not related to any known mammalian lectins. Surprisingly, MR60 was found to be identical to ERGIC-53 (Schindler, R., Itin, C., Zerial, M., Lottspeich, F., and Hauri, H. P. (1993) Eur. J. Cell Biol. 61, 1-9), a type I integral membrane protein, defined as a marker of the intermediate compartment that recycles between the Golgi apparatus and endoplasmic reticulum; MR60/ERGIC-53 shares with VIP-36 significant homologies with leguminous plant lectins (Fiedler, K., and Simmons, R. (1994) Cell 77, 625-626). We extend these findings in evidencing a structural homology between MR60/ERGIC-53 and mammalian galectins (soluble beta galactose binding proteins). MR60/ERGIC-53 is the first lectin characterized as an endoplasmic reticulum-Golgi protein. Accordingly, this intracellular mannose binding protein could be involved in the traffic of glycoproteins between endoplasmic reticulum and the Golgi apparatus.