Chromosomes of mouse embryos and newborn young: preparations from membranes and tail tips.
Chromosomes of mouse embryos and newborn young: preparations from membranes and tail tips.
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小鼠胚胎和新生幼仔的染色体：膜和尾尖的制备物。
DOI：
10.3109/10520297209116541
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
C. E. Ford
中科院分区：
文献类型：
--
作者：
E. P. Evans;M. Burtenshaw;C. E. Ford
染色体制剂可以从中期小鼠胚胎的膜中获得，从而可以保存胚胎本身以用于其他研究。将整个植入物从妊娠子宫中解剖出来，并在 20-25°C 下暴露于 TC 培养基 199 中的 2 × 10−7 M Colcemid 中 1 小时。取出膜，根据胎龄置于相同温度下的 1% Na3-柠檬酸盐中 4-12 分钟，在 3-4 ml 3:1 乙醇-乙酸混合物中固定 1 分钟至 3 小时，然后转移到0.5 ml 60% 乙酸水溶液 4 分钟。细胞从膜上脱离进入乙酸。然后通过在加热的载玻片上干燥乙酸来获得染色体涂片。该方法可以针对新生幼崽的尾尖进行修改。妊娠第8至18天的胚胎和1至6天龄的小鼠是合适的。 （第 8 至 10 天，将胚胎和膜结合起来制备制剂。）可以在 90 分钟内从胎膜中获得准备好进行评分的制剂，在 150 分钟内从尾尖中获得准备好的制剂。
Chromosome preparations can be obtained from the membranes of midterm mouse embryos allowing the embryos themselves to be preserved for other investigations. Whole implants are dissected from the gravid uterus and exposed to 2 × 10−7 M Colcemid in TC medium 199 for 1 hr at 20–25 C. The membranes are removed, placed in 1% Na3-citrate at the same temperature for 4–12 min depending on gestational age, fixed in 3–4 ml of a 3:1 ethanol-acetic acid mixture for 1 min to 3 hr and transferred to 0.5 ml aqueous 60% acetic acid for 4 min. Cells detach from the membranes into the acetic acid. Chromosome spreads are then obtained by drying the acetic acid on heated slides. The method can be modified for tail tips of newborn young. Embryos on the 8th to 18th day of gestation and 1- to 6-day-old mice are suitable. (On the 8th to 10th days, preparations are made from embryo and membranes combined.) Preparations ready for scoring can be obtained from foetal membranes in 90 min and from tail tips in 150 min.