A tomato (Solanum lycopersicum) APETALA2/ERF gene, SlAP2a, is a negative regulator of fruit ripening
A tomato (Solanum lycopersicum) APETALA2/ERF gene, SlAP2a, is a negative regulator of fruit ripening
复制标题
DOI：
10.1111/j.1365-313x.2010.04384.x
复制
发表时间：
2010-12-01
期刊：
影响因子：
7.2
通讯作者：
Giovannoni, James
中科院分区：
文献类型：
--
作者：
Chung, Mi-Young;Vrebalov, Julia;Giovannoni, James
肉质果实从成熟到成熟的转变是由外源和内源信号调节的，这些信号协调果实向对种子传播生物有吸引力的最终状态的转变。番茄是生物学和遗传学调节特定成熟途径的模型，包括乙烯、类胡萝卜素和细胞壁代谢，以及上游信号和转录调节因子。迄今为止所描述的成熟相关转录因子包括RIN-MADS、CLEAR NON-RIPENING、TAGL1和LeHB-1基因都编码成熟现象的正调控因子。在这里，我们描述了一个APETALA2转录因子（SlAP2a），通过果实成熟的转录分析鉴定，在番茄果实成熟过程中诱导，并负调控。RNAi抑制SlAP2a导致果实产生乙烯过量，早熟，并通过改变类胡萝卜素途径通量改变类胡萝卜素积累谱。这些结果表明，SlAP2a在正常的番茄果实成熟过程中作为成熟活性调节剂发挥作用，并平衡正成熟调节剂的活性。
P>The transition of fleshy fruit maturation to ripening is regulated by exogenous and endogenous signals that coordinate the transition of the fruit to a final state of attractiveness to seed dispersing organisms. Tomato is a model for biology and genetics regulating specific ripening pathways including ethylene, carotenoids and cell wall metabolism in addition to upstream signaling and transcriptional regulators. Ripening-associated transcription factors described to date including the RIN-MADS, CLEAR NON-RIPENING, TAGL1 and LeHB-1 genes all encode positive regulators of ripening phenomena. Here we describe an APETALA2 transcription factor (SlAP2a) identified through transcriptional profiling of fruit maturation that is induced during, and which negatively regulates, tomato fruit ripening. RNAi repression of SlAP2a results in fruits that over-produce ethylene, ripen early and modify carotenoid accumulation profiles by altering carotenoid pathway flux. These results suggest that SlAP2a functions during normal tomato fruit ripening as a modulator of ripening activity and acts to balance the activities of positive ripening regulators.