Cell Wall Peptidoglycan Mutants of Escherichia coli K-12: Existence of Two Clusters of Genes, mra and mrb, for Cell Wall Peptidoglycan Biosynthesis
Cell Wall Peptidoglycan Mutants of Escherichia coli K-12: Existence of Two Clusters of Genes, mra and mrb, for Cell Wall Peptidoglycan Biosynthesis
复制标题
大肠杆菌 K-12 的细胞壁肽聚糖突变体：用于细胞壁肽聚糖生物合成的两个基因簇 mra 和 mrb 的存在
作者：
T. Miyakawa;H. Matsuzawa;M. Matsuhashi;Y. Sugino
分离到了大肠杆菌K-12在42℃下不能形成细胞壁肽聚糖的温度敏感突变体。对热敏步骤进行了生化表征，并对肽聚糖合成酶的编码基因进行了定位。这些基因分为两组：一组位于leu和azi之间约1.5 min处，被命名为mra (murein a)；第二组位于距argH和metB约77.5 min的位置，命名为mrb （murein b，顺序为metB-argH-mrb）。基因定位与细胞壁肽聚糖生物合成过程中所涉及的酶的顺序和定位没有简单的关系。
Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis.