The effects of adrenocorticotropin, guanylylimidodiphosphate, dibutyryl adenosine 3',5'-monophosphate, and exogenous substrates on corticosteroid output by ovine fetal adrenal cells at different times in pregnancy.
The effects of adrenocorticotropin, guanylylimidodiphosphate, dibutyryl adenosine 3',5'-monophosphate, and exogenous substrates on corticosteroid output by ovine fetal adrenal cells at different times in pregnancy.
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促肾上腺皮质激素、鸟苷酰亚胺二磷酸、二丁酰腺苷3,5-单磷酸和外源性底物对妊娠不同时间绵羊胎儿肾上腺细胞皮质类固醇输出的影响。
DOI：
10.1210/endo-111-3-889
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
J. Challis
中科院分区：
文献类型：
--
作者：
L. Manchester;J. Challis
为了检测妊娠期间绵羊胎儿肾上腺反应性变化的相关因素，我们将妊娠50、100和130天及足月的绵羊胎儿肾上腺细胞与ACTH（1 - 24）（一种GTP类似物[鸟苷5（1）-（β，γ-亚氨基）三磷酸]，二丁酰cAMP，和皮质类固醇产生的潜在底物，双烯醇酮，孕酮，17 α-羟基双烯醇酮，和17 α-羟孕酮（17 OHP 4）。我们测定了这些细胞在体外孵育过程中皮质醇（F）、皮质酮（B）和孕酮（P4）的产量。促肾上腺皮质激素刺激F和B输出的第50天和在足月，但不是在中期妊娠。所有浓度ACTH的平均F与B输出比从第50天的3.3上升到足月时的11.3。GTP类似物在足月时刺激F、B和P4输出，但在第100或130天不刺激。双丁酰cAMP刺激的F和B输出在第50天，并在长期和刺激的P4输出在所有阶段的妊娠检查。外源性17OHP 4在妊娠期的任何时候都转化为F。P4向F和17OHP 4向F的相对转化率（转化商）从第100天的0.09上升到足月时的0.80。P4在妊娠期的所有时间点均转化为B。在足月时，从δ 4底物（P4和17 OHP 4）形成的F大于从δ 5底物（β烯醇酮和17 α OH-β烯醇酮）形成的F。结果表明：（1）促肾上腺皮质激素（ACTH）刺激的F分泌在孕50天和足月妊娠时最高，（2）孕中期的F分泌减少可能与GTP和cAMP生成缺陷和17 α-羟化酶活性降低有关;和3）足月，17 α-羟化酶似乎比妊娠中期对F产生的限制更少，但δ 5，3 β-羟基类固醇脱氢酶可能更是如此。
To examine the factors responsible for the changing responsiveness of ovine fetal adrenals during pregnancy, we incubated dispersed adrenal cells obtained from fetal sheep on days 50, 100, and 130 of pregnancy and at term with ACTH (1--24), a GTP analog [guanosine 5(1)-(beta, gamma-imido)triphosphate], dibutyryl cAMP, and potential substrates for corticosteroid production, pregnenolone, progesterone, 17 alpha-hydroxypregnenolone, and 17 alpha-hydroxyprogesterone (17OHP4). We determined the output of cortisol (F), corticosterone (B) and progesterone (P4) by these cells during in vitro incubation. ACTH stimulated F and B output on day 50 and at term, but not in midpregnancy. The mean F to B output ratio for all concentrations of ACTH rose from 3.3 on day 50 to 11.3 at term. The GTP analog stimulated F, B, and P4 output at term, but not on day 100 or 130. Dibutyryl cAMP stimulated F and B output on day 50 and at term and stimulated P4 output at all stages of pregnancy examined. Exogenous 17OHP4 was converted to F at all times in pregnancy. The relative conversion of P4 to F and 17OHP4 to F (conversion quotient) rose from 0.09 on day 100 to 0.80 at term. P4 was converted to B at all times in gestation. At term, formation of F from delta 4 substrates (P4, and 17OHP4) was greater than that from delta 5 substrates (pregnenolone and 17 alpha OH-pregnenolone). We conclude that (1) the output of F in response to ACTH was highest on day 50 and at term pregnancy; 2) the loss of response in midpregnancy may be associated with a defect in GTP and cAMP generation and with reduced 17 alpha-hydroxylase activity; and 3) a term, 17 alpha-hydroxylase appears to be less limiting to F production than in midgestation, but delta 5, 3 beta-hydroxysteroid dehydrogenase may be more so.
胎儿人肾上腺皮质的调节：促肾上腺皮质激素对胎儿和定形区细胞单层培养物的生长和功能的影响。
影响因子：
4.8
作者：
Simonian,MH;Gill,GN
通讯作者：
Gill,GN
灵长类动物胎儿肾上腺和性腺的调节和功能。
DOI：
10.1016/b978-0-12-571137-1.50007-4
发表时间：
1981
期刊：
Recent progress in hormone research
影响因子：
--
作者：
Jaffe,RB;Seron-Ferre,M;Crickard,K;Koritnik,D;Mitchell,BF;Huhtaniemi,IT
通讯作者：
Huhtaniemi,IT