Gamma-radiation-induced crosslinking of cell-specific chromosomal nonhistone protein-DNA complexes in HeLa chromatin.
Gamma-radiation-induced crosslinking of cell-specific chromosomal nonhistone protein-DNA complexes in HeLa chromatin.
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伽玛辐射诱导 HeLa 染色质中细胞特异性染色体非组蛋白蛋白-DNA 复合物的交联。
DOI：
10.2307/3575602
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发表时间：
1981
影响因子：
3.4
通讯作者：
L. Hnilica
中科院分区：
文献类型：
--
作者：
R. Oliński;R. Briggs;L. Hnilica
用去组织的HeLa细胞染色质注射兔或山羊获得特异性抗血清。对分离的染色质进行γ射线照射（10至100 krad）增加了其与这些特异性抗血清的免疫反应性。相反，照射分离的染色体非组蛋白蛋白质或DNA，然后重建，导致重建复合物的免疫反应性几乎完全丧失。选择性蛋白质解离实验表明，辐射交联的活性非组蛋白的DNA。交联蛋白的聚丙烯酰胺凝胶电泳进一步证实了这一解释。
Specific antisera were obtained by injecting rabbits or goats with dehistonized HeLa cell chromatin. Gamma irradiation (10 to 100 krad) of the isolated chromatin increased its immunological reactivity with these specific antisera. Conversely, irradiation of the isolated chromosomal nonhistone proteins or DNA followed by reconstitution resulted in a nearly complete loss of immunological reactivity of the reconstituted complexes. Selective protein dissociation experiments indicated that radiation crosslinked the active nonhistone proteins to the DNA. This interpretation was further substantiated by polyacrylamide gel electrophoresis of the crosslinked proteins.
HeLa 细胞周期中的核抗原。
影响因子：
4.3
作者：
Banjar,ZM;Briggs,RC;Hnilica,LS;Stein,JL;Stein,GS
通讯作者：
Stein,GS