Disturbed vagal nerve distribution in embryonic chick hearts after treatment with all-trans retinoic acid
Disturbed vagal nerve distribution in embryonic chick hearts after treatment with all-trans retinoic acid
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DOI：
10.1007/s004290050150
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发表时间：
1998-05-01
期刊：
影响因子：
--
通讯作者：
Poelmann, RE
中科院分区：
文献类型：
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作者：
Broekhuizen, MLA;Gittenberger-de Groot, AC;Poelmann, RE
用全胚标本和维甲酸处理的鸡胚连续切片研究迷走神经的分布。白色来航鸡胚在第15阶段用1 μ g全反式视黄酸（n=11）或溶剂二甲基亚砜（假手术胚胎，n=8）处理。8个胚胎作为正常对照。在第34阶段，用解剖显微镜检查所有27个胚胎。为了揭示迷走神经模式，取出心脏并用HNK-1抗体进行整体染色。在维甲酸处理组的三个心脏中发现了形态学的心内异常-右心室双出口-为了深入探索心脏中的迷走神经分布，单独设计了一组维甲酸胚胎（n=5）、假手术胚胎（n=4）和对照胚胎（n=5）的心脏，仅用于连续切片和用HNK-1抗体染色。维甲酸处理的胚胎的所有心脏都表现出在心脏表面和心脏壁内的迷走神经分布紊乱。与对照组相比，假手术胚胎的迷走神经模式没有改变。它的结论是，视黄酸干扰迷走神经图案的发展，无论同时存在的心内畸形。其机制和功能意义仍有待研究。
The distribution of the vagal nerve was studied in whole-mount specimens and serial sections of chick embryos after retinoic acid treatment. White Leghorn chick embryos were treated at stage 15 either with 1 mu g all-trans retinoic acid (n=11), or with the solvent dimethylsulphoxide (sham-operated embryos, n=8). Eight embryos served as normal controls. At stage 34 all 27 embryos were examined with a dissecting microscope. In order to reveal the vagal patterning, the hearts were removed and whole-mount stained with the HNK-1 antibody. In three hearts of the retinoic acid-treated group a morphologic intracardiac anomaly - a double outlet right ventricle - was found. To explore in depth the vagal nerve distribution in the heart, a separate set of hearts of retinoic acid embryos (n=5), sham-operated (n=4) and control embryos (n=5), was devised solely for serial sectioning and staining with the HNK-1 antibody. All hearts of retinoic acid-treated embryos showed a disturbed vagal nerve distribution both over the surface of the heart and within the heart wall. The vagal patterning was not altered in the sham-operated embryos compared to controls. It is concluded that retinoic acid disturbs the development of vagal nerve patterning regardless of the concurrent presence of intracardiac malformations. The mechanism and functional implications remain to be investigated.