Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison
Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison
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DOI：
10.1007/s10142-004-0104-3
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发表时间：
2004-05-01
影响因子：
2.9
通讯作者：
Appels, Rudi
中科院分区：
文献类型：
--
作者：
Li, Chengdao;Ni, Peixiang;Appels, Rudi
收获前发芽给世界各地的粮食工业造成了重大的经济损失。种子缺乏足够的休眠是在潮湿天气条件下田间收获前发芽的主要原因。虽然这种特性是由多个基因控制的，但它也是高度遗传的。在大麦染色体5 H长臂上发现了一个控制穗发芽和种子休眠的主效QTL，它可以解释70%以上的表型变异。利用比较基因组学方法在大麦、小麦和水稻中鉴定控制种子休眠的候选基因，从而鉴定收获前发芽的一个方面。大麦种子休眠/穗发芽QTL位于水稻3号染色体长臂末端的一个同线性良好的区域。对水稻基因组DNA序列进行了注释，在5 HL上发现了一个控制种子休眠/穗发芽QTL的候选基因，即GA 20氧化酶基因。该染色体区域也与小麦染色体4AL的端粒区域共享同线性，但位于小麦种子休眠性QTL之外。小麦4号染色体的AL QTL与水稻3号和11号染色体均同线。在这两种情况下，相应的QTL种子休眠已被定位在水稻。
Pre-harvest sprouting results in significant economic loss for the grain industry around the world. Lack of adequate seed dormancy is the major reason for pre-harvest sprouting in the field under wet weather conditions. Although this trait is governed by multiple genes it is also highly heritable. A major QTL controlling both pre-harvest sprouting and seed dormancy has been identified on the long arm of barley chromosome 5H, and it explains over 70% of the phenotypic variation. Comparative genomics approaches among barley, wheat and rice were used to identify candidate gene(s) controlling seed dormancy and hence one aspect of pre-harvest sprouting. The barley seed dormancy/pre-harvest sprouting QTL was located in a region that showed good synteny with the terminal end of the long arm of rice chromosome 3. The rice DNA sequences were annotated and a gene encoding GA20-oxidase was identified as a candidate gene controlling the seed dormancy/pre-harvest sprouting QTL on 5HL. This chromosomal region also shared synteny with the telomere region of wheat chromosome 4AL, but was located outside of the QTL reported for seed dormancy in wheat. The wheat chromosome 4AL QTL region for seed dormancy was syntenic to both rice chromosome 3 and 11. In both cases, corresponding QTLs for seed dormancy have been mapped in rice.