Epigenetic differentiation and relationship to adaptive genetic divergence in discrete populations of the violet Viola cazorlensis
Epigenetic differentiation and relationship to adaptive genetic divergence in discrete populations of the violet Viola cazorlensis
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DOI：
10.1111/j.1469-8137.2010.03298.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Bazaga, Pilar
中科院分区：
文献类型：
--
作者：
Herrera, Carlos M.;Bazaga, Pilar
在植物中，基于DNA甲基化的表观遗传变异通常是可遗传的，并可能影响进化过程。在这一假设可以被评估之前，关于表观遗传变异的基本问题仍然需要在现实世界的背景下解决，包括它的大小，自然群体内和之间的结构，以及与遗传背景相关的自主性。胞嘧啶甲基化的程度和模式，以及与群体间适应性遗传分歧的关系，调查了西班牙南部紫罗兰Viola cazorlensis（堇菜科）使用甲基化敏感扩增多态性（MSAP）技术，基于同裂限制性内切酶对位点的敏感性差异，对扩增片段长度多态性方法（AFLP）进行了修改V.cazorlensis植物的基因组表现出广泛的甲基化水平，并且基于甲基化的表观遗传变异被构造成不同的群体间和群体内组分。群体的表观遗传分化与AFLP数据的贝叶斯群体基因组分析所揭示的适应性遗传分化相关。适应性AFLP位点与甲基化敏感MSAP位点的甲基化状态在个体基因组水平上存在显著关联，表观遗传和遗传个体变异相关选择的群体特异性差异模式可以解释表观遗传-遗传适应性群体分化的协同作用。
In plants, epigenetic variations based on DNA methylation are often heritable and could influence the course of evolution. Before this hypothesis can be assessed, fundamental questions about epigenetic variation remain to be addressed in a real-world context, including its magnitude, structuring within and among natural populations, and autonomy in relation to the genetic context.Extent and patterns of cytosine methylation, and the relationship to adaptive genetic divergence between populations, were investigated for wild populations of the southern Spanish violet Viola cazorlensis (Violaceae) using the methylation-sensitive amplified polymorphism (MSAP) technique, a modification of the amplified fragment length polymorphism method (AFLP) based on the differential sensitivity of isoschizomeric restriction enzymes to site-specific cytosine methylation.The genome of V. cazorlensis plants exhibited extensive levels of methylation, and methylation-based epigenetic variation was structured into distinct between- and within-population components. Epigenetic differentiation of populations was correlated with adaptive genetic divergence revealed by a Bayesian population-genomic analysis of AFLP data. Significant associations existed at the individual genome level between adaptive AFLP loci and the methylation state of methylation-susceptible-MSAP loci.Population-specific, divergent patterns of correlated selection on epigenetic and genetic individual variation could account for the coordinated epigenetic-genetic adaptive population differentiation revealed by this study.