Correlation between μCT imaging, histology and functional capacity of the osteoarthritic knee in the rat model of osteoarthritis.
Correlation between μCT imaging, histology and functional capacity of the osteoarthritic knee in the rat model of osteoarthritis.
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DOI：
10.1186/s12967-015-0641-7
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发表时间：
2015-08-25
影响因子：
7.4
通讯作者：
Wilkie D
中科院分区：
文献类型：
--
作者：
Bagi CM;Zakur DE;Berryman E;Andresen CJ;Wilkie D
为了获得对人类关节炎的最有意义的理解，必须选择可转化为人类条件的疾病模型和方法。本研究的主要目的是评价许多分析技术和生物标志物在骨关节炎（OA）的内侧髁撕裂（MMT）模型中准确测量骨和软骨形态和代谢的能力。在大鼠中进行MMT手术以诱导OA。采用动态负重系统（DWB）评价大鼠前、后肢的负重能力。在10周研究结束时，通过显微计算机断层扫描（μCT）、对比增强μCT（EPIC μCT）成像和传统组织学评价软骨病理学。评价胫骨干骺端和骨骺的骨组织，包括软骨下骨。组织学技术和动态组织形态计量学用于评估软骨形态和骨矿化。研究结果表明MMT手术对手术肢体的承重能力有负面影响。手术导致胫骨平台内侧关节软骨严重和广泛恶化，血清、EPIC μCT和组织学中的CTX-II升高证明了这一点。μCT骨分析显示受损软骨下的软骨下骨增厚，干骺端松质骨丢失和活跃骨赘形成。该研究强调需要使用各种相互补充的方法，以全面了解OA在器官，组织和细胞水平上的病理生理学。本研究的结果表明，使用组织学、μCT和EPIC μCT以及功能DWB测试提供了强大的组合，可全面评估OA的关键方面并增强数据解释。
To acquire the most meaningful understanding of human arthritis, it is essential to select the disease model and methodology translatable to human conditions. The primary objective of this study was to evaluate a number of analytic techniques and biomarkers for their ability to accurately gauge bone and cartilage morphology and metabolism in the medial meniscal tear (MMT) model of osteoarthritis (OA). MMT surgery was performed in rats to induce OA. A dynamic weight bearing system (DWB) system was deployed to evaluate the weight-bearing capacity of the front and hind legs in rats. At the end of a 10-week study cartilage pathology was evaluated by micro computed tomography (μCT), contrast enhanced μCT (EPIC μCT) imaging and traditional histology. Bone tissue was evaluated at the tibial metaphysis and epiphysis, including the subchondral bone. Histological techniques and dynamic histomorphometry were used to evaluate cartilage morphology and bone mineralization. The study results showed a negative impact of MMT surgery on the weight-bearing capacity of the operated limb. Surgery caused severe and extensive deterioration of the articular cartilage at the medial tibial plateau, as evidenced by elevated CTX-II in serum, EPIC μCT and histology. Bone analysis by μCT showed thickening of the subchondral bone beneath the damaged cartilage, loss of cancellous bone at the metaphysis and active osteophyte formation. The study emphasizes the need for using various methodologies that complement each other to provide a comprehensive understanding of the pathophysiology of OA at the organ, tissue and cellular levels. Results from this study suggest that use of histology, μCT and EPIC μCT, and functional DWB tests provide powerful combination to fully assess the key aspects of OA and enhance data interpretation.