Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions.
Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions.
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DOI：
10.1002/ange.201600593
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发表时间：
2016-04-04
期刊：
影响因子：
--
通讯作者：
Zhou D
中科院分区：
文献类型：
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作者：
Guo Y;Sakonsinsiri C;Nehlmeier I;Fascione MA;Zhang H;Wang W;Pöhlmann S;Turnbull WB;Zhou D
开发了一种用于制备紧凑、致密的多价甘露糖封端的量子点（QD）的高效帽交换方法。所得到的量子点已成功地用于探测HIV/埃博拉病毒受体DC-SIGN和DC-SIGNR（统称为DC-SIGN/R）的多价相互作用，使用灵敏的比率Förster共振能量转移（FRET）测定。QD探针特异性结合DC-SIGN，但不结合其密切相关的受体DC-SIGNR，这通过其特异性阻断DC-SIGN与埃博拉病毒糖蛋白的结合而得到进一步证实。调整QD表面甘露糖价揭示了DC-SIGN更有效地结合密集堆积的甘露糖苷。基于FRET的热力学研究表明，结合是焓驱动的。这项工作建立了量子点FRET作为一种快速，灵敏的技术探测结构和热力学的多价蛋白质配体相互作用。
A highly efficient cap‐exchange approach for preparing compact, dense polyvalent mannose‐capped quantum dots (QDs) has been developed. The resulting QDs have been successfully used to probe multivalent interactions of HIV/Ebola receptors DC‐SIGN and DC‐SIGNR (collectively termed as DC‐SIGN/R) using a sensitive, ratiometric Förster resonance energy transfer (FRET) assay. The QD probes specifically bind DC‐SIGN, but not its closely related receptor DC‐SIGNR, which is further confirmed by its specific blocking of DC‐SIGN engagement with the Ebola virus glycoprotein. Tuning the QD surface mannose valency reveals that DC‐SIGN binds more efficiently to densely packed mannosides. A FRET‐based thermodynamic study reveals that the binding is enthalpy‐driven. This work establishes QD FRET as a rapid, sensitive technique for probing structure and thermodynamics of multivalent protein–ligand interactions.