GmNAC181 promotes symbiotic nodulation and salt tolerance of nodulation by directly regulating GmNINa expression in soybean
GmNAC181 promotes symbiotic nodulation and salt tolerance of nodulation by directly regulating GmNINa expression in soybean
复制标题
GmNAC181通过直接调节大豆GmNINa表达促进共生结瘤和结瘤耐盐性
DOI：
10.1111/nph.18343
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发表时间：
2022-07-15
期刊：
影响因子：
9.4
通讯作者：
Li, Xia
中科院分区：
文献类型：
--
作者：
Wang, Xiaodi;Chen, Kuan;Li, Xia
大豆（Glycine max）是世界上最重要的作物之一。在低氮条件下，大豆可以与根瘤菌形成共生关系，获得足够的氮素供其生长和生产。结瘤信号控制大豆与根瘤菌的共生。大豆根瘤菌起始（GmNINa）基因是大豆根瘤菌形成的重要调控因子。然而，GmNINa的转录调控在很大程度上仍然未知。结瘤对盐胁迫敏感，但其机制尚不清楚。在这里，我们确定了NAC转录因子GmNAC181（也称为GmNAC11）作为GmNSP1a的相互作用蛋白。GmNAC181在大豆中的过表达或敲低分别导致根瘤数量的增加或减少。相应地，GmNINa的表达分别大大上调和下调。此外，我们还发现GmNAC181可以直接结合到GmNINa启动子上以激活其基因表达。有趣的是，GmNAC181在盐胁迫下高度诱导并促进共生体的形成。我们鉴定了结瘤途径中的一个新的转录激活因子GmNINa，并揭示了在盐胁迫条件下，GmNAC181作为网络节点协调GmNINa的表达和共生结瘤的机制。
Soybean (Glycine max) is one of the most important crops world-wide. Under low nitrogen (N) condition, soybean can form a symbiotic relationship with rhizobia to acquire sufficient N for their growth and production. Nodulation signaling controls soybean symbiosis with rhizobia. The soybean Nodule Inception (GmNINa) gene is a central regulator of soybean nodulation. However, the transcriptional regulation of GmNINa remains largely unknown. Nodulation is sensitive to salt stress, but the underlying mechanisms are unclear. Here, we identified an NAC transcription factor designated GmNAC181 (also known as GmNAC11) as the interacting protein of GmNSP1a. GmNAC181 overexpression or knockdown in soybean resulted in increased or decreased numbers of nodules, respectively. Accordingly, the expression of GmNINa was greatly up- and downregulated, respectively. Furthermore, we showed that GmNAC181 can directly bind to the GmNINa promoter to activate its gene expression. Intriguingly, GmNAC181 was highly induced by salt stress during nodulation and promoted symbiotic nodulation under salt stress. We identified a new transcriptional activator of GmNINa in the nodulation pathway and revealed a mechanism by which GmNAC181 acts as a network node orchestrating the expression of GmNINa and symbiotic nodulation under salt stress conditions.