Erythropoietin expression in primary rat Sertoli and peritubular myoid cells
Erythropoietin expression in primary rat Sertoli and peritubular myoid cells
复制标题
DOI：
10.1182/blood.v98.9.2872
复制
发表时间：
2001-11-01
期刊：
影响因子：
20.3
通讯作者：
Gassmann, M
中科院分区：
文献类型：
--
作者：
Magnanti, M;Gandini, O;Gassmann, M
肾脏和肝脏是促红细胞生成素(EPO)合成的主要器官。然而，已在脑、肺和睾丸等几个器官中检测到EPO信使RNA(MRNA)。此外，包括大鼠间质细胞在内的不同细胞类型的EPO受体均有功能。本研究的目的是鉴定表达EPO mRNA的睾丸细胞，并用竞争性逆转录聚合酶链式反应(RT-PCR)定量检测其表达水平。除整个睾丸外，EPO在支持细胞和管周肌样细胞中均有表达，而在间质细胞中未检测到信号。支持细胞暴露于CoCl2后，EPO基因表达水平升高。半定量竞争性逆转录聚合酶链式反应显示卵泡刺激素刺激的支持细胞EPO基因表达水平升高，而睾酮作用于肾小管周围肌样细胞可降低EPO基因表达。由于血-睾丸屏障，EPO的基础表达提示这种激素在睾丸中的功能尚未明确。(C)2001年，由美国血液病学会提供。
Kidney and liver are the major organs of erythropoietin (Epo) synthesis. However, Epo messenger RNA (mRNA) has been detected in several organs, such as brain, lung, and testis. Furthermore, functional Epo receptors have been demonstrated on different cell types, including rat Leydig cells. The aim of the study was to identify testicular cells expressing Epo mRNA and to quantitate its levels by competitive reverse transcriptase-polymerase chain reaction (RT PCR). Besides whole testis, Epo transcripts were found in Sertoli and peritubular myoid cells, while no signal was detected in Leydig cells. Exposure of Sertoli cells to CoCl2 led to an increase of Epo mRNA level. Semiquantitative competitive RT PCR presented an increase in the level of Epo mRNA in sertoli cells stimulated by follicle-stimulating hormone, while exposure of peritubular myoid cells cultures to testosterone reduced Epo mRNA expression. Due to the blood-testis barrier, basal expression of Epo suggests a not yet defined function of this hormone in testis. (C) 2001 by The American Society of Hematology.